南京农业大学学报2025，Vol.48Issue(3)：548-559,12.DOI:10.7685/jnau.202402011
番红花不同部位代谢组及黄酮类成分分析
Analysis of metabolome and flavonoids of different parts in Crocus sativus
摘要
Abstract
[Objectives]The paper aimed to compare the metabolite and flavonoid contents of different parts of Crocus sativus in order to provide scientific basis for the development and utilization of C.sativus non-medicinal resources.[Methods]The petals,pistils and stamens of C.sativus was conducted by LC-MS technology as test materials,and non-targeted metabolomics analysis of the samples.At the same time,a colorimetric method was used to determine the total flavonoid content in different parts,and ultra-performance liquid chromatography(UPLC)was used to determine the relatively high flavonoid content.[Results]The results showed that 851,825,and 877 metabolites were identified in petals,stamens and pistils,respectively;there were 769 metabolites in total among the three parts,and there were 4 unique metabolites in pistils and stamens;there were no unique metabolites in petals.The differential metabolites were mainly carboxylic acids and their derivatives,oxygen-containing organic compounds,and flavonoids.The number of up-regulated and down-regulated metabolites in petals relative to pistils were 237 and 251,respectively,among which 7 of the top 15 up-regulated compounds were flavonoids,and the differential metabolites were significantly enriched,in the flavonoid biosynthesis and flavonoid and flavonol biosynthesis pathways,mainly including kaempferol 3-O-glucoside,6-hydroxyflavonoids,quercetin,delphinidin,luteolin,diosmetin,etc.The colorimetric results showed that the flavonoids in the floral organs of crocus were mainly distributed in the petals.[Conclusions]Different parts of C.sativus were rich in metabolites,especially the petals were rich in flavonoids.关键词
番红花/非靶向代谢组学/超高效液相色谱/(UPLC)/黄酮Key words
Crocus sativus/non-targeted metabolomics/ultra-performance liquid chromatography(UPLC)/flavonoids分类
中医学引用本文复制引用
杨朋三,徐丹丹,刘小兰,孙莉琼,唐晓清,王康才,张凯..番红花不同部位代谢组及黄酮类成分分析[J].南京农业大学学报,2025,48(3):548-559,12.基金项目
中央高校基本科研业务费专项资金(KYZZ2022002) （KYZZ2022002）
南京农业大学-西藏农牧学院联合基金项目(KYYZ2023001) （KYYZ2023001）
企业合作项目(2021-1230) （2021-1230）
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023293) （zyyzdxk-2023293）
