南京农业大学学报2025，Vol.48Issue(3)：604-611,8.DOI:10.7685/jnau.202402005
牛疱疹病毒4型TL01株的分离鉴定及遗传进化分析
Isolation,identification and genetic analysis of a bovine herpesvirus 4 isolate TL01
张心琴 1戴鑫钧 1姚火春1
作者信息
- 1. 南京农业大学动物医学院,江苏 南京 210095||南京农业大学世界动物卫生组织猪链球菌病参考实验室,江苏 南京 210095
- 折叠
摘要
Abstract
[Objectives]The aim of this study was to isolate and identify a novel bovine herpesvirus 4(BoHV-4)strain in China,associated with reproductive disorders in large-scale dairy herds.[Methods]Swabs were collected from the reproductive tracts of cattle suspected to be infected with BoHV-4 at a cattle farm in the Northeast China.Positive samples were inoculated with HeLa cells for virus isolation,followed by identification using specific qPCR,indirect immunofluorescence(IFA),and electron microscopy.One step growth curve of the virus was determined.The gB and TK genes of the virus were amplified through PCR and analyzed for genetic evolution.[Results]Positive samples were inoculated with cells.After three generations of transmission,the cells exhibited typical cytological effects(CPE)characteristic of herpesviruses such as round contraction and aggregation.IFA results revealed distinct green fluorescence specifically in infected cells.Electron microscopy images showed infected cells with a diameter ranging of 100-150 nm,displaying clear capsid and capsule structures.The isolated strain named TL01 demonstrated a growth curve peaking at 36 hours post-infection(106.3 TCID50·mL-1).PCR amplification confirmed that gB gene belonged to genotype 2 along with reference strain DN599 while TK gene belonged to genotype 1 alongside reference strain Movar33/63.[Conclusions]This study successfully isolated one BoHV-4 strain,providing a reference for further studies on the biological characterization and epidemiology of BoHV-4.关键词
牛疱疹病毒4型/病毒分离鉴定/遗传进化分析Key words
bovine herpesvirus 4/virus isolation and identification/genetic evolution analysis分类
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张心琴,戴鑫钧,姚火春..牛疱疹病毒4型TL01株的分离鉴定及遗传进化分析[J].南京农业大学学报,2025,48(3):604-611,8.
