南京中医药大学学报2025，Vol.41Issue(5)：637-651,15.DOI:10.14148/j.issn.1672-0482.2025.0637
基于UPLC-Q-TOF-MS鉴定玉屏风散入血成分
Identification of the Components of Yu-Ping-Feng-San Absorbed into Plasma Based on UPLC-Q-TOF-MS
摘要
Abstract
OBJECTIVE To analyze the plasma-entering components of the ethanol extract of Yu-Ping-Feng-San by ultra-per-formance liquid chromatography-quadrupole-time of flight tandem mass spectrometry(UPLC-Q-TOF-MS)technology,so as to pro-vide a reference for the study of the pharmacological substances of Yu-Ping-Feng-San.METHODS Male BALB/c mice were ga-vaged with the ethanol extract of Yu-Ping-Feng-San(6.5 g·kg-1),and plasma samples were collected before administration(0 h)and 1,2,4,6,8,and 12 h after administration.The UPLC-Q-TOF-MS technology was used to analyze and identify the components absorbed into the plasma.RESULTS A total of 73 chemical components in the ethanol extract of Yu-Ping-Feng-San were identi-fied,and 26 plasma-entering components were identified in all plasma samples.Among the plasma-entering components,11 compo-nents were from Saposhnikoviae Radix,and 15 components were from Radix Astragali.CONCLUSION The chemical components and plasma-entering components of the ethanol extract of Yu-Ping-Feng-San were analyzed comprehensively,which provides a reference for further clarifying the pharmacological substance basis and mechanism of action of Yu-Ping-Feng-San.关键词
玉屏风散/乙醇提物/超高效液相色谱-四极杆-飞行时间串联质谱法/成分鉴定/入血成分/防风/黄芪Key words
Yu-Ping-Feng-San/ethanol extract/ultra-performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry/component identification/plasma-entering components/Saposhnikoviae Radix/Radix Astragali分类
医药卫生引用本文复制引用
黄福满,宋亚军,左童文,李琳,郑云枫,郑劼,洪敏..基于UPLC-Q-TOF-MS鉴定玉屏风散入血成分[J].南京中医药大学学报,2025,41(5):637-651,15.基金项目
国家自然科学基金面上项目(82074087,82274168,82374111) （82074087,82274168,82374111）
江苏高校"青蓝工程" （）
江苏省中医药科技发展计划重点项目(ZD201902) （ZD201902）
江苏省高等学校自然科学研究重大项目(20KJA360005) （20KJA360005）
江苏省研究生科研与实践创新计划项目(KYCX24_2277) （KYCX24_2277）
