海南热带海洋学院学报2025，Vol.32Issue(2)：1-10,10.DOI:10.13307/j.issn.2096-3122.2025.02.01
虫黄藻(Effrenium voratum)对高温胁迫的生理响应
Physiological Response of Effrenium voratum to High-temperature Stress
摘要
Abstract
Global warming poses a great threat to the survival of corals.In recent years,coral bleaching events have occurred frequently,and the death or loss of the coral symbiotic zooxanthellae is the major factor of coral bleaching.The tolerance of coral zooxanthellae to high-temperature stress is the key to determining the threshold of coral bleaching.Using the isolated cultured Effrenium voratum as the research object,the molecular characterization of coral zooxanthellae was first carried out,and followed by the high-temperature stress experiment.The ambient temperature of the control group was set at a constant temperature of 26℃,and that of the experimental group was set at 29℃and 32℃,respectively,to study the physiological effects of seawater warming on the growth,Chl a concentration,superoxide dismutase(SOD)and catalase(CAT)activity of E.voratum under the background of global warming.The high-throughput sequencing results showed that the relative abundance of E.voratum in the isolated culture algal solution was(99.971±0.032)%.The results of high-temperature stress experiments showed that the growth of E.voratum was inhibited when exposed to 29℃.Still,they managed to maintain the trend of increasing density,Chl a increased and maintained a relatively high level within a short period,and the activities of the antioxidant enzymes SOD and CAT exhibited a continuous upward trend.When E.voratum was exposed to 32℃,the density of E.voratum showed a negative increase,Chl a showed a rise and then decreased to a level lower than that of the control group,SOD activity showed a rise and then decreasing trend,and CAT activity showed a trend of continuous increase.Therefore,E.voratum may have certain adaptation potential at the tem-perature range of 26～29℃,while at 32℃,high temperature stress will lead to cell damage or even death of E.voratum.关键词
虫黄藻/Effrenium voratum/高温胁迫/生理响应/离体培养/高通量测序Key words
coral zooxanthellae/Effrenium voratum/high temperature stress/physiological response/in vitro culture/high throughput sequencing分类
生物学引用本文复制引用
张廷超,潘能斌,唐媛媛,何燃,蓝梦玲,韦丽菲,覃祯俊..虫黄藻(Effrenium voratum)对高温胁迫的生理响应[J].海南热带海洋学院学报,2025,32(2):1-10,10.基金项目
广西自然科学基金青年基金项目(2022GXNSFBA035449) （2022GXNSFBA035449）
国家自然科学基金项目(42206157) （42206157）
大学生创新创业训练计划项目(202310593497) （202310593497）
