海南热带海洋学院学报2025，Vol.32Issue(2)：32-45,14.DOI:10.13307/j.issn.2096-3122.2025.02.04
高体鰤Na+/K+-ATPase基因家族的生物信息学分析及其在盐度胁迫下的表达
Bioinformatic Analysis of Na+/K+-ATPase Gene Family of Greater Amberjack(Seriola dumerili)and Their Expression under Salinity Stress
摘要
Abstract
In order to identify the Na+/K+-ATPase gene family members of greater amberjack(Seriola dumerili),and to detect the mRNA expression levels of each Na+/K+-ATPase gene family member of greater amberjack(Seriola du-merili)under different salinity stress,and to reveal the molecular response of Na+/K+-ATPase genes to salinity change in the external water environment,firstly,the mRNA and amino acid sequences of the Na+/K+-ATPase gene family member were downloaded from the database and the family members were subjected to identification,phylogenetic analysis,syn-teny analysis,gene structure analysis,and protein conserved motif analysis;Secondly,qRT-PCR(quantitative real-time polymerase chain reaction)was used to detect the mRNA expression levels of Na+/K+-ATPase genes in the gills and kid-ney of greater amberjack under different salinity stress.The results showed that a total of 12 Na+/K+-ATPase gene family members(atp1a1a.4,atp1a1b,atp1a2a,atp1a3a,atp1a3b,atp1b1a,atp1b1b,atp1b2a,atp1b2b,atp1b3a,atp1b3b and atp1b4)in greater amberjack were identified.Phylogenetic analysis showed that members of Na+/K+-ATPase gene family in greater am-berjack were clustered individually with other teleosts,and then clustered with their counterparts in the tetrapods(hu-mans,mice,birds,reptiles,amphibians).The results of synteny analysis showed that the members of the Na+/K+-ATPase gene family and their upstream and downstream genes had good covariance.Meanwhile,the gene structure and protein motifs of Na+/K+-ATPase gene family members were highly similar and conserved.The transcriptomic and qRT-PCR results showed that the expression level of atp1b1b mRNA in gills was significantly up-regulated at 72 h under acute hyper-salinity stress.In contrast,the expression level of atp1b1a mRNA in gills decreased with the increase of sa-linity under the chronic salinity stress for 30 days.In addition,the expression level of atp1b1b mRNA in kidney of greater amberjack in the hyper-salinity group was significantly lower than that in the hypo-salinity group,while the expres-sion level of atp1b3b mRNA in hypo-salinity group was significantly downregulated in comparison with that in the control group.The aforementioned results suggest that β1 subunit in gills and the β1 and β3 subunits in kidney of greater amber-jack are mainly involved in osmoregulation in response to hypo-,and hyper-salinity stress.These results are of reference significance for the study of the evolution of the Na+/K+-ATPase gene family in fish,and lay the foundation for further research on the function of the Na+/K+-ATPase genes in greater amberjack.关键词
高体鰤/Na+/K+-ATPase/基因家族/盐度胁迫/基因表达水平Key words
greater amberjack/Na+/K+-ATPase/gene family/salinity stress/gene expression level分类
水产学引用本文复制引用
周彤,李欣怡,茹笑影,袁嘉慧,黄洋,朱春华,李广丽,石红娟..高体鰤Na+/K+-ATPase基因家族的生物信息学分析及其在盐度胁迫下的表达[J].海南热带海洋学院学报,2025,32(2):32-45,14.基金项目
广东海洋大学第一批"揭榜挂帅"项目(030301022301) （030301022301）
广东省"扬帆计划"现代化海洋牧场产业人才振兴计划子项目(HYJ-YFJH-2024001) （HYJ-YFJH-2024001）
湛江市科技计划项目(2024R1010) （2024R1010）
南方海洋科学与工程广东省实验室(湛江)资助项目(ZJW-2023-10) （湛江）
广东海洋大学创新训练计划项目(CXXL2022001) （CXXL2022001）
