热带亚热带植物学报2025，Vol.33Issue(3)：241-252,12.DOI:10.11926/jtsb.4887
'茗科1号'茶鲜叶转录和代谢轮廓的模式识别研究
Pattern Recognition of Transcriptomic and Metabolic Profiles in Tea Shoots of'Mingke 1'
摘要
Abstract
To delve deeper into the unique attributes of fresh leaves from oolong tea varieties,the green tea cultivar'Fudingdahao'with one bud and two or three leaves was used as the control(CK),the compositional differences at both the transcriptional and metabolic levels between two or three leaves with a bud(TM)and two to four leaves with a banjhi bud(MM)from the highly fragrant oolong tea variety'Mingke 1'were analyzed.The results showed that both tea variety and harvesting standards significantly influenced the transcriptomic and metabolic profiles of tea shoots.Notably,the transcriptional and metabolic profiles of CK,TM,and MM exhibited distinct and well-defined pattern differentiations.Enrichment and correlation analysis of differentially expressed genes and metabolites revealed that TM vs CK and MM vs TM displayed distinct GO functional enrichment patterns significant in molecular functions,cellular components,and biological processes.The former had more entries in biological processes and fewer entries in cellular components.Additionally,"Biosynthesis of various plant secondary metabolites","Arginine and proline metabolism",along with"Carotenoid biosynthesis","Phenylpropanoid biosynthesis"and"Glycine,serine,and threonine metabolism"were identified as the top 20 shared KEGG-enriched metabolic pathways in the two omics analyses of TM vs CK and MM vs TM.In TM vs CK,there was a significant decrease in the abundance of L-phenylalanine,trans-cinnamic acid,and 4-guani-dinobutyric acid,while in MM vs TM,there was a significant increase in choline abundance and a significant decrease in the abundance of L-phenylalanine,L-tryptophan,and L-homoserine.All of these changes were closely associated with significant upregulation or downregulation of multiple genes in the respective enriched metabolic pathways.Additionally,the significant increase in abscisate abundance in MM vs TM was highly correlated with significant upregulation of violaxanthin de-epoxidase and lycopene β-cyclase genes in the carotenoid biosynthesis pathway.So,these would provide the necessary reference for further elucidating the biological characteristics of fresh leaves obtained from'Mingke 1'and their versatility in producing multiple types of tea.关键词
乌龙茶/鲜叶/转录组/代谢组/模式识别Key words
Oolong tea/Fresh leaf/Transcriptome/Metabolome/Pattern recognition引用本文复制引用
陈林,项丽慧,宋振硕,陈键,张应根..'茗科1号'茶鲜叶转录和代谢轮廓的模式识别研究[J].热带亚热带植物学报,2025,33(3):241-252,12.基金项目
福建省自然科学基金项目(2023J01197) （2023J01197）
福建省属公益类科研院所基本科研专项(2022R1029001,2023R1027003) （2022R1029001,2023R1027003）
福建省农业科学院科技创新团队项目(CXTD2021004-2)资助 This work was supported by the Project for Natural Science in Fujian(Grant No.2023J01197),the Special Project for Public Welfare Research Institutes in Fujian(Grant No.2022R1029001,2023R1027003),and the Project for Scientific and Technological Innovation Team of Fujian Academy of Agricultural Sciences(Grant No.CXTD2021004-2). （CXTD2021004-2）
