水产科技情报2025，Vol.52Issue(3)：137-146,10.DOI:10.16446/j.fsti.20240200121
美洲鲥脑转录组多态性EST-SNP标记的开发与利用
Development and application of polymorphic EST-SNP markers from the brain transcriptome of Alosa sapidissima
摘要
Abstract
A total of 113 898 single nucleotide polymorphism(EST-SNP)markers based on expression sequence tags were successfully identified from the brain transcriptome data of Alosa sapidissima by bioinformatics tools,and 57 polymorphic EST-SNP markers were developed for genetic diversity assessment in A.sapidissima.The minor allele frequency(MAF),the observed heterozygosity(Ho),expected heterozygosity(He)and polymorphism information content(PIC)were 0.067-0.483,0.033-0.567,0.124-0.499 and 0.117-0.375,respectively.Two loci of the 57 EST-SNP significantly deviated from the Hardy-Weinberg equilibrium(P<0.05).The results demonstrated that these polymorphic EST-SNP markers were potent tools for evaluating genetic diversity and germ-plasm analysis of A.sapidissima,which could be capitalized in future conservation strategies and breeding pro-grams to protect and improve this valuable fish.关键词
美洲鲥/转录组/单核苷酸多态性/遗传多样性Key words
Alosa sapidissima/transcriptome/single nucleotide polymorphism/genetic diversity引用本文复制引用
于爱清,施永海,徐嘉波,刘永士,严银龙..美洲鲥脑转录组多态性EST-SNP标记的开发与利用[J].水产科技情报,2025,52(3):137-146,10.基金项目
上海市科技兴农重点攻关项目(2021-02-08-00-12-F00788) （2021-02-08-00-12-F00788）
上海领军人才项目(沪人社专2021-67号) （沪人社专2021-67号）
上海长江口主要经济水生动物人工繁育工程技术研究中心项目(13DZ2251800). （13DZ2251800）
