四川中医2025，Vol.43Issue(5)：103-110,8.DOI:10.3969/j.issn.1000-3649.sczy2501100007
基于"心肾相关"理论研究三黄一龙汤调控水通道蛋白防治佐剂性关节炎模型大鼠心肌纤维化的机制
Based on theory of"heart-kidney correlation",to study the mechanism of Sanhuang Yilong Decoction regulating aquaporins and preventing myocardial fibrosis in adjuvant arthritis model rats
摘要
Abstract
Objective Based on the"heart-kidney correlation"theory,this article explores the improving effect and mechanism of Sanhuang Yilong Decoction(SYD)on cardiovascular injury in AA model.Methods Seventy Wistar female rats were randomly divided into contral group,AA group,SYD low,medium and high dose groups(19.8g/kg,39.6g/kg,79.2g/kg),leflunomide group(2.1mg/kg)and placebo group(equal volume of normal saline).Complete Freund's adjuvant(CFA)was injected subcutaneously into all rats except the contral group to establish AA model.After continuous intragastric administration for 4 weeks,the serum,heart and kidney tissues of rats in each group were collected.Masson staining was used to observe the degree of cardiac fibrosis,enzyme-linked immunosorbent assay was used to detect peripheral blood AQP1,AQP2 and AVP levels,Western blotting and immunofluorescence staining were used to detect AQP1 levels in heart tissue,and kidney Tissue AVP and AQP2 protein level.Results Masson staining results showed that the heart tissue of AA rats showed fibrotic changes,mainly characterized by blue collagen fiber deposition,the levels of FGF23,α-SMA and FN protein were significantly increased.SYD can significantly reduce the fibrosis changes in cardiac tissue,decrease the levels of FGF23,α-SMA and FN protein,and down-regulate the expression of AQP1 in cardiac tissue and AVP and AQP2 in renal tissue(P<0.05).Conclusion SYD can inhibit the activity of AVP,reduce the production and transport of AQP2 in the kidney,inhibit the expression of aquaporin AQP1 in the heart,reduce water retention in the body,down-regulate the expression of FGF23,α-SMA and FN proteins related to promoting myocardial fibrosis,and alleviate the cardiac injury and myocardial fibrosis induced by AA.This reflects the theory of"heart-kidney correlation"in TCM.关键词
佐剂性关节炎/三黄一龙汤/心肾相关/心血管损伤/水通道蛋白Key words
Adjuvant arthritis/Sanhuang Yilong Decoction/Heart-Kidney correlation/Cardiovascular injury/Aquaporin分类
医药卫生引用本文复制引用
刘德芳,杨羚,罗婷,张耀雷,晏姣..基于"心肾相关"理论研究三黄一龙汤调控水通道蛋白防治佐剂性关节炎模型大鼠心肌纤维化的机制[J].四川中医,2025,43(5):103-110,8.基金项目
四川省应用基础研究基金重点研发项目(2019YFS0198). （2019YFS0198）
