四川中医2025，Vol.43Issue(5)：182-192,11.DOI:10.3969/j.issn.1000-3649.sczy2501020004
基于系统生物学与Box-Behnken响应面法探究六味地黄丸(汤)多成分协同作用及煎煮方案优化
Investigation of the multi-component synergistic effects and decoction optimization of Liuwei Dihuang Tang using systems biology and Box-Behnken response surface methodology
郭浩林 1尹平阳 1杨欣玥 1李金泉 2王元琪 2李卫红 2王臻 2王鹏 3卞旭东 4肖雅霖 2李寒 1刘艺 1张凡云2
作者信息
- 1. 北京中医药大学中医学院,北京 100029
- 2. 北京中医药大学护理学院,北京 100029
- 3. 北京中医药大学中药学院,北京 100029
- 4. 北京中医药大学东直门医院,北京 100700
- 折叠
摘要
Abstract
Objective This study integrates systems biology with the Box-Behnken response surface methodology to optimize the extraction process of Liuwei Dihuang Decoction(LWD)and explore its multi-component synergistic mechanisms,providing a scientific basis for the treatment of hyperthyroidism.Methods Principles,morroniside,paeonol,and loganin were identified as the primary evaluation indicators.The Box-Behnken response surface methodology was employed,with water volume,extraction time,and extraction frequency as variables,combined with the AHP-entropy weight method to optimize the extraction process.Results The optimal extraction parameters were determined to be:8 times the water volume,1.5 hours per extraction,and three extractions in total.The study showed that the main components affect core targets such as ALB,IL6,TNF,INS,and IL1B,thereby exerting therapeutic effects on hyperthyroidism.Conclusion This study successfully optimized the extraction process of LWD,ensuring its stability and feasibility.It provides an experimental foundation and quality improvement reference for further research and clinical application,promoting the use and development of traditional Chinese herbal formulas in modern medicine.关键词
六味地黄汤/提取工艺优化/系统生物学/信息熵权法/Box-Behnken响应面法Key words
Liuwei Dihuang Tang/Extraction process optimization/Systems biology/Entropy weight method/Box-Behnken response surface methodology分类
医药卫生引用本文复制引用
郭浩林,尹平阳,杨欣玥,李金泉,王元琪,李卫红,王臻,王鹏,卞旭东,肖雅霖,李寒,刘艺,张凡云..基于系统生物学与Box-Behnken响应面法探究六味地黄丸(汤)多成分协同作用及煎煮方案优化[J].四川中医,2025,43(5):182-192,11.
