山东农业科学2025，Vol.57Issue(4)：1-13,13.DOI:10.14083/j.issn.1001-4942.2025.04.001
板栗2-ODD基因家族的鉴定及其在干旱胁迫下的表达分析
Identification of Chinese Chestnut 2-ODD Gene Family and Their Expression Analysis under Drought Stress
摘要
Abstract
The 2-ODD gene family is the second largest enzyme family in the plant and has an important role in regulating plant response to stresses.Using bioinformatics methods,we identified 123 Cm2-ODD genes from the chestnut genome,their chromosomal location,protein physicochemical properties,conserved motifs,gene structure,collinear genes and cis-acting elements,and the expression under drought stress were ana-lyzed.The results found that all the Cm2-ODD family proteins contained the conserved 2OG-Fe Ⅱ_Oxy and DIOX_N domains,and were hydrophilic proteins.Most members of Cm2-ODD gene family had conserved mo-tifs of Motif 1 to Motif 9,and only a part members had Motif 10,indicating that the function of Cm2-ODD pro-teins might be relatively conserved.Collinearity analysis found that the Cm2-ODD family genes within the chestnut genome might be under purifying selection,and a massive replication of the 2-ODD gene family might occur after the differentiation of monocotyledonous and dicotyledonous plants.Codon preference analysis showed that Cm2-ODD family genes preferred to end with A or U.The result of cis-acting element analysis showed that Cm2-ODD family genes contained more abscisic acid and drought response elements,speculating that its function might be associated with drought stress.Meanwhile,transcriptome analysis proved that the ex-pression levels of 13 members of the Cm2-ODD gene family were significantly affected by drought stress,a-mong which,the expression of Cm2-ODD19 and Cm2-ODD16 genes was up-regulated.关键词
板栗/Cm2-ODD基因家族/生物信息学分析/干旱胁迫/基因表达Key words
Chinese chestnut/Cm2-ODD gene family/Bioinformatics analysis/Drought stress/Gene expression分类
园艺学与植物营养学引用本文复制引用
曾扬鹃,杨涌,郭江辉,张海娥,曹飞..板栗2-ODD基因家族的鉴定及其在干旱胁迫下的表达分析[J].山东农业科学,2025,57(4):1-13,13.基金项目
国家重点研发计划项目"主要经济林优质高产新品种创制与精准栽培技术"(2022YFD2200400) （2022YFD2200400）
板栗产业技术教育部工程研究中心项目(PT2022-01) （PT2022-01）
