山东农业科学2025，Vol.57Issue(4)：14-21,8.DOI:10.14083/j.issn.1001-4942.2025.04.002
河北杨PhMYB14基因的克隆及生物信息学分析
Cloning and Bioinformatics Analysis of PhMYB14 Gene in Populus hopeiensis
摘要
Abstract
MYB transcription factors play important roles in plant growth and development in ways of in-teractions,signal transduction,and gene expression regulation.In this study,using the seedlings of Populus hopeiensis as materials,PhMYB14 gene was cloned,its bioinformatic analysis was performed,and its tissue-specific expression in P.hopeiensis was analyzed.The results showed that the length of coding sequence(CDS)of PhMYB14 gene was 723 bp,encoding 240 amino acid residues.The PhMYB14 protein had the mo-lecular weight of 27 512.87 Da and the theoretical isoelectric point of 5.17,and was an unstable hydrophilic protein with no signal peptide and transmembrane structure.Homologous sequence alignment and protein phy-logenetic tree analysis showed that PhMYB14 was the most closely related to Populus trichocarpa and Populus alba,both of which also belonged to the genus Populus,followed by Salix sinopurpurea and Salix suchowensis.Prediction of subcellular localization suggested that PhMYB14 was mainly locatted in chloroplasts.The promot-er region of PhMYB14 contained various cis-acting elements including photoresponsive elements and methyl jasmonate response elements.Real-time fluorescence quantitative analysis(qRT-PCR)showed that PhMYB14 was expressed in the roots,stems and leaves of poplar seedlings with the highest expression level in leaves.The results of this study could provide a reference for further study of the functional mechanism of PhMYB14 in the resistance to diseases and insect pests of P.hopeiensis.关键词
河北杨/PhMYB14/基因克隆/生物信息学/表达分析Key words
Populus hopeiensis/PhMYB14/Gene Cloning/Bioinformatics/Expression analysis分类
林学引用本文复制引用
庞琳琳,张骐,代金玲,白玉娥..河北杨PhMYB14基因的克隆及生物信息学分析[J].山东农业科学,2025,57(4):14-21,8.基金项目
国家科技重大专项"转基因杨树新品种培育及产业化研究"任务(2018ZX08020002-005-005) （2018ZX08020002-005-005）
内蒙古自治区研究生科研创新项目(S20231104Z) （S20231104Z）
