山东农业科学2025，Vol.57Issue(4)：22-31,10.DOI:10.14083/j.issn.1001-4942.2025.04.003
藜麦ANT基因家族的鉴定及其在愈伤组织中的表达分析
Genome-Wide Identification of ANT Gene Family in Quinoa(Chenopodium quinoa Willd.)and Their Expression Analysis in Callus Tissues
摘要
Abstract
ANT/euANT(AINTEGUMENTA)is a class of APETALA2(AP2)type transcription factor that only exists in the plant and belongs to the AP2/EREBP family.In this study,the identification and bioin-formatics analysis of quinoa ANT gene family were carried out utilizing the genome database of quinoa,and their expression in different callus tissues and buds were analyzed through qRT-PCR experiment.The results indicated that a total of 13 members of the ANT gene family with two AP2 conserved domains were identified,which unevenly distributed on 9 chromosomes of quinoa and were named as CqANT1 to CqANT13 based on their positions on the chromosomes.The CqANT1 to CqANT13 proteins had the amino acid length ranging from 244 to 710 aa,the molecular weight from 27.20 to 77.22 kDa,and the isoelectric point from 5.57 to 9.21,so they were classified as unstable hydrophilic proteins localized in nucleus.Gene collinearity analysis revealed that 12 CqANT genes formed 6 pairs of segmental duplications,but no tandem duplication events were identi-fied.The analysis of promoter cis-acting elements found that the promoter regions of quinoa ANT family mem-bers contained various elements response to hormones and environmental factors,suggesting their involvement in regulating quinoa's responses to abiotic stresses and hormone signaling cascades.They had high homology with that of the dicotyledonous plant Arabidopsis.Expression analysis showed that the expression of the ANT genes in quinoa were tissue-specific and had a certain response to abiotic stresses.The expression of the quinoa ANT genes were present in both bud and different callus tissues,indicating that the quinoa ANT genes played important roles in induction and maintenance of buds and callus tissues.The results of this study could provide a basis for further revealing the function and regulatory mechanism of quinoa ANT genes in the future.关键词
藜麦/ANT基因家族/生物信息学分析/愈伤组织表达Key words
Quinoa(Chenopodium quinoa)/ANT gene family/Bioinformatics analysis/Expression in callus tissue分类
农业科学引用本文复制引用
高爱红,张侠,曹萌,安珂欣,尹海波,郭善利,张平,赵波..藜麦ANT基因家族的鉴定及其在愈伤组织中的表达分析[J].山东农业科学,2025,57(4):22-31,10.基金项目
山东省重点研发计划(农业良种工程品种培育)项目(2023LZGC011) （农业良种工程品种培育）
烟台市校地融合发展项目 （）
