山东农业科学2025，Vol.57Issue(4)：46-50,5.DOI:10.14083/j.issn.1001-4942.2025.04.006
小麦茎基腐病相关基因TaDIR-B1的分子检测及遗传效应分析
Molecular Detection and Genetic Effect Analysis of TaDIR-B1 Gene Associated with Fusarium Crown Rot in Wheat
摘要
Abstract
Fusarium crown rot(FCR)has become one of the most important diseases in the main wheat production region of China.Detecting the distribution of FCR resistance alleles and analyzing their genetic effects could provide guidance and available resources for wheat FCR resistance breeding.TaDIR-B1 gene has been reported to be involved in the regulation of wheat lignin synthesis,whose loss-of-function could signifi-cantly improve lignin synthesis,and then improve the resistance to FCR.In the present study,a total of 106 wheat varieties(lines)originated from home and abroad were used as materials,the disease severity was in-vestigated by inoculation test with FCR in field,and the distribution of different variants and their genetic effects on FCR were analyzed by TaDIR-B1 sequencing.The results showed that among the 106 materials,73 belonged to mutant type(TaDIR-B1b)which accounting for 68.87％of the total,and 33 belonged to wild type(TaDIR-B1a)which accounting for 31.13％of the total.The mean disease severity of FCR in the materi-als carrying TaDIR-B1b was lower than that in the materials carrying TaDIR-B1a,and the differences in three environments and the mean value were significant(P＜0.05)or extremely significant.The proportion of Ta-DIR-B1b materials in Shandong and Hebei Province was relatively higher.Jimai 1703,Mantol,Baimangmai,Shi 4185,Boomer and Azulon carried TaDIR-B1a gene and had lower disease severity of FCR,which could be used as parents for resistant variety breeding.关键词
小麦/茎基腐病/遗传效应/分子标记Key words
Wheat/Fusarium crown rot/Genetic effect/Molecular marker分类
植物保护学引用本文复制引用
赵琪,郭灿,程敦公,李法计,李豪圣..小麦茎基腐病相关基因TaDIR-B1的分子检测及遗传效应分析[J].山东农业科学,2025,57(4):46-50,5.基金项目
山东省重点研发计划(重大科技创新工程)项目(2022LZGC005 （重大科技创新工程）
2021LZGC009) （）
济南市"新高校20条"资助项目(202228067) （202228067）
国家现代农业产业技术体系项目(CARS-03-6) （CARS-03-6）
