山东农业科学2025，Vol.57Issue(4)：51-58,8.DOI:10.14083/j.issn.1001-4942.2025.04.007
基于重测序的开农系列花生品种(系)遗传多样性分析
Genetic Diversity Analysis of Kainong Series Peanut Varieties(Lines)Based on Whole-Genome Resequencing
摘要
Abstract
In this study,a total of 30 Kainong series peanut varieties(lines)derived from 0317 combi-nation(Kainong 30 × Kaixuan 016)with larger promotion areas were used as materials,and the genetic simi-larity analysis,population structure analysis and cluster analysis based on the second-generation whole genome resequencing data were conducted,in order to provide basic materials and visual technical references for effi-cient utilization of peanut germplasm resources in China.The results showed that after data control,423 149 high-quality single nucleotide polymorphism(SNP)sites were obtained that were evenly distributed on the chromosomes.Among them,chromosome 3 had the most SNP sites and chromosome 10 had the least,account-ing for 8.56％and 2.66％of the total,respectively.There were 435 genetic similarity coefficient values be-tween different peanut varieties(lines)ranging from 0.55 to 0.96,and the average value was 0.73.The high-est genetic similarity coefficient(0.96)was between Kainong 301 and Kainong 306,showing the closest ge-netic relationship.The lowest genetic similarity coefficient(0.55)was between Kainong 176 and Kainong 100,showing the farthest genetic relationship.Through population structure analysis,the 30 peanut varieties(lines)were divided into three subgroups,accounting for 30.00％,33.33％and 36.67％of the total,respec-tively.Among which,the genetic backgrounds of Kainong 99 and Kainong 65 were relatively complex.The cluster analysis results showed that the result from population genetic heatmap of the 30 peanut varieties(lines)was consistent with that from evolutionary tree;the 30 peanut varieties(lines)were divided into three groups;combined with the pedigree relationships,it was found that varieties bred from the same combination might not necessarily clustered in the same group.In conclusion,the genetic similarity among the 30 peanut varieties(lines)in this study was relatively high,so the genetic basis should be broadened.In the process of excellent peanut varieties breeding,it might be possible to comprehensively analyze pedigree and SNP-based clustering results for screening germplasm resources.关键词
花生/种质资源/0317组合/单核苷酸多态性/遗传多样性Key words
Peanut/Germplasm resources/0317 combination/Single nucleotide polymorphism/Genetic diversity分类
农业科学引用本文复制引用
郭敏杰,邓丽,苗建利,雒峰,殷君华,孙宜根,肖婷,任丽..基于重测序的开农系列花生品种(系)遗传多样性分析[J].山东农业科学,2025,57(4):51-58,8.基金项目
国家花生产业技术体系项目(CARS-13) （CARS-13）
河南省农业良种联合攻关项目(20220100001) （20220100001）
开封市科技计划重点研发专项(22ZDYF008) （22ZDYF008）
