山东农业科学2025，Vol.57Issue(4)：77-88,12.DOI:10.14083/j.issn.1001-4942.2025.04.010
外源褪黑素对盐碱胁迫下大豆叶绿素代谢的影响
Effects of Exogenous Melatonin on Chlorophyll Metabolism of Soybean under Saline-Alkali Stress
摘要
Abstract
Saline-alkali stress(SA)is a kind of common abiotic stress in nature that can inhibit the ab-sorption of water and nutrients by plants,induce plant cells to produce a large number of reactive oxygen spe-cies(ROS),and inhibit plant growth and biomass accumulation.As an active molecule with various regulatory abilities,melatonin(MT)plays the important role in regulating seed germination and plant growth and devel-opment under stresses.In this study,the soybean varieties HH49(with saline-alkali tolerance)and HN95(with saline-alkali sensitivity)were used as experimental materials,and their seedlings were cultured in sa-line-alkali soil(pH=8.78)to V1 stage by potted method.Foliar application of exogenous 300 µmol·L-1 MT was conducted to clarify the effects of MT treatment on chlorophyll metabolism and photosynthetic capacity of soybean leaves under SA.The results showed that compared with the control(CK),SA increased the contents of superoxide anion(O2·-)and hydrogen peroxide(H2O2)in soybean seedling leaves,regulated the expres-sion levels of genes related to chlorophyll(Chl)synthesis and decomposition in soybean seedling leaves,de-creased chlorophyll content and photosynthesis and fluorescence parameters,affected the accumulation and metabolism of carbohydrates,and inhibited plant growth.Exogenous MT treatment significantly increased the activity of antioxidant enzymes in leaves,decreased the ROS accumulation,up-regulated the expression levels of GmGSA2,GmGUN4,GmPORB and GmCHLG genes related to chlorophyll synthesis,down-regulated the ex-pression levels of GmHCAR,GmPPH,GmPAO and GmRCCR genes related to chlorophyll decomposition,in-creased the content of chlorophyll in leaves,improved the photosynthetic capacity of plants,promoted the ac-cumulation and metabolism of soluble sugar,sucrose and starch,and so promoted plant growth of soybean.The results of this study could provide a theoretical basis for breeding saline-tolerant soybean varieties and develo-ping saline-alkali tolerant tillage measures.关键词
大豆(Glycine max L.)/褪黑素/ROS积累与清除/叶绿素合成与分解/光合作用Key words
Soybean(Glycine max L.)/Melatonin/ROS accumulation and removal/Chlorophyll syn-thesis and decomposition/Photosynthesis分类
农业科技引用本文复制引用
马悦,丁绪,胡嘉宸,潘祺丰,余洋,赵强,周伟,杜吉到..外源褪黑素对盐碱胁迫下大豆叶绿素代谢的影响[J].山东农业科学,2025,57(4):77-88,12.基金项目
黑龙江省自然科学基金项目"外源褪黑素调控盐碱胁迫下大豆光合碳代谢的作用机制研究"(LH2023C078) （LH2023C078）
黑龙江省"双一流"新一轮建设学科协同创新成果建设项目"耐盐碱大豆种质的应用及抗盐碱栽培技术的示范"(LJGXCG2022-111) （LJGXCG2022-111）
黑龙江八一农垦大学研究生创新科研项目"外源褪黑素对盐碱胁迫下大豆叶片叶绿素代谢途径的影响机制"(NXYYJSCX2022-Y06) （NXYYJSCX2022-Y06）
