山西农业科学2025，Vol.53Issue(3)：16-26,11.DOI:10.3969/j.issn.1002-2481.2025.03.03
谷子LEA基因家族全基因组鉴定及表达分析
Genome-Wide Identification and Expression Analysis of LEA Gene Family in Foxtail Millet
摘要
Abstract
In order to explore the characteristics and function of the foxtail millet LEA gene family,based on the published Arabidopsis thaliana LEA protein sequence,LEA family members were genome-widely identified using bioinformatics methods.Subsequent analysis were conducted on the physical and chemical properties,conserved motifs,phylogenetic tree,cis-regulatory elements,collinearity,and expression patterns of the proteins encoded by SiLEAs.These results showed that a total of 33 SiLEA members were identified in the foxtail millet LEA family,and SiLEAs were unevenly distributed on 9 chromosomes.Subcellular prediction found that SiLEAs were located in the nucleus,cytoplasm,chloroplast,and mitochondria.Through phylogenetic tree analysis,different subfamilies were divided into three groups,and most of those SiLEAs gene in the same subfamily showed similar gene structure and conserved motif component characteristics.Cis-regulatory element analysis showed that there were many response elements related to plant hormones and stress in the promoter region of SiLEAs,Inter-species collinearity analysis found that the number of gene pairs,which were collinear with monocotyledon,were much higher than those with dicotyledon.Analysis of the gene expression patterns of SiLEAs revealed that SiLEA3-6,SiLEA3-7,SiDHN-4,SiDHN-1,and SiLEA3-8 were highly expressed in roots,stems,leaves,and before flowering and seven days after flowering,and these five genes also all contained the elements related to growth and development,suggesting that SiLEA3-6,SiLEA3-7,SiDHN-4,SiDHN-1,and SiLEA3-8 might be the key genes of LEA gene family in response to growth and development in foxtail millet.关键词
谷子/LEA基因家族/生物信息学/系统发育树/顺式调控元件/共线性Key words
foxtail millet/LEA gene family/bioinformatics/phylogenetic tree/cis-regulatory elements/collinearity分类
农业科技引用本文复制引用
宋梦瑶,李娜,秦家范,郭冰,李春霞,李君霞,马小倩..谷子LEA基因家族全基因组鉴定及表达分析[J].山西农业科学,2025,53(3):16-26,11.基金项目
河南省重点研发专项(231111110300) （231111110300）
国家自然科学基金(32301841) （32301841）
河南省自然科学基金(242300421320) （242300421320）
河南科技大学A类博士人才项目(13480103) （13480103）
