山西农业科学2025，Vol.53Issue(3)：27-43,17.DOI:10.3969/j.issn.1002-2481.2025.03.04
不同种植物中HMGR基因的生物信息学分析
Bioinformatics Analysis of HMGR Genes in Different Plants
摘要
Abstract
To identify HMGR(3-Hydroxy-3-methylglutaryl coenzyme A reductase)genes in different plants,in this study,bioinformatics methods were used to analyze the physicochemical properties of HMGR protein in 18 plants including Arabidopsis,tomato,and potato.The characteristics of their transmembrane structure,subcellular localization,and modification sites were analyzed.The higher-order and conserved domains of the proteins encoded by HMGR genes in 18 plants were predicted.The results indicate that the HMGR protein sequence of melon was the longest,consisting of 1 244 amino acids,while the HMGR protein sequence of strawberry was the shortest,consisting of 131 amino acids.The isoelectric point of the HMGR protein in corn was the highest,at 10.12,and that of strawberry was the lowest,at 4.72.Except for mango,corn,wheat,poplar,and peach,the HMGR proteins of other plants carried a negative charge.Except for corn,poplar,and strawberry,the aliphatic index of the HMGR proteins of other plants were above 90,suggesting that most HMGR proteins were hydrophobic.The HMGR protein was the most stable in mango.The strongest hydrophobicity was the highest in pear and apricot.The strongest hydrophilicity was the highest in poplar.Except for strawberry,HMGRs in other plants had transmembrane structures,with the highest number of transmembrane structure locations in melon.The analysis of protein modification sites showed that the HMGR protein in strawberry had the fewest glycosylation sites.Apricot had the most glycosylation modification sites.Arabidopsis had the highest number of phosphorylation sites,while strawberry had the lowest number of phosphorylation sites.The secondary structure was mainly composed of α-helices and random coils.The HMGR gene was divided into 3 classes and the HMGR protein of 17 plants,except for corn,contained the HMG-CoA reductase Class I domain.关键词
HMGR基因/生物信息学/理化性质/基因编码蛋白/保守结构域Key words
HMGR gene/bioinformatics analysis/physicochemical properties/gene-encoded proteins/conserved domains分类
农业科技引用本文复制引用
李娜,樊裕璇,韩红艳,宋宇琴,李六林,赵红梅..不同种植物中HMGR基因的生物信息学分析[J].山西农业科学,2025,53(3):27-43,17.基金项目
山西省高等学校科技创新项目(2023L313) （2023L313）
山西省基础研究计划(自由探索类)(202303021222261) （自由探索类）
