上海农业学报2025，Vol.41Issue(2)：1-7,7.DOI:10.15955/j.issn1000-3924.2025.02.01
水稻蔗糖非酵解相关基因OsSnRK1s的自然变异分析
Natural mutation analysis of sucrose non-fermentation related gene OsSnRK1s in rice
摘要
Abstract
Using bioinformatics methods,natural mutation analysis was conducted on the promoter and transcription regions of the two α subunit genes OsSnRK1.1 and OsSnRK1.2 of the sucrose non-formentation related gene SnRK1,which senses energy signals in rice cells.The results showed that there were 24 and 47 natural mutation sites in the promoter and transcription regions of OsSnRK1.1 gene,respectively.Among them,there were 3 natural mutation sites in the exons,and only 1 non-synonymous mutation was found in exon 9.The mutation at this site resulted in a change from proline at position 278 to serine(P→S278),located between the N-terminal catalytic domain and the ubiquitin related domain.This mutation could well distinguish indica and japonica rice.There were 46 and 30 natural mutation sites in the promoter and transcription regions of OsSnRK1.2 gene,respectively.Among them,there were 6 natural mutation sites in the exon of OsSnRK1.2 gene,including 5 non-synonymous natural mutation sites.Haplotype analysis was conducted on 4 726 publicly available rice material sequencing data using the 6 natural mutation sites.The results showed that the OsSnRK1.2 gene was mainly divided into 3 haplotypes:haplotype Ⅰ(TGGGGA),haplotype Ⅱ(CGGGGA),and haplotype Ⅲ(TGAGGG).Among them,97％of rice materials belonged to haplotype Ⅰ,and the mutations in haplotypes Ⅱand Ⅲ were the isoleucine mutation at position 47 to threonine(I→T47)and the arginine mutation at position 354 to histidine(R→H354),respectively.Three haplotypes involved 2 natural mutation sites,while the remaining 3 natural mutation sites had a low frequency in the rice population.Although there was a significant difference in the number of natural mutation sites of OsSnRK1.1 and OsSnRK1.2 genes encoding the α subunit,the OsSnRK1.1 gene had shown greater conservation during evolution.This study is of great significance for understanding the sequence information of OsSnRK1 gene and its encoded protein,providing important theoretical basis for further exploring its phosphorylation activity and biological functions.关键词
水稻/自然群体/OsSnRK1基因/能量平衡/单倍型分析Key words
Rice/Natural populations/OsSnRK1 gene/Energy balance/Haplotype analysis分类
农业科技引用本文复制引用
张余,谢炳峰,马孝松,赵洪,吴成鑫,刘昆,叶水烽,陈海荣..水稻蔗糖非酵解相关基因OsSnRK1s的自然变异分析[J].上海农业学报,2025,41(2):1-7,7.基金项目
上海市农业科学院质标所创新团队建设计划(植物种质评价与保存zbcx第1-3号) （植物种质评价与保存zbcx第1-3号）
