上海农业学报2025，Vol.41Issue(2)：8-15,8.DOI:10.15955/j.issn1000-3924.2025.02.02
基于CRISPR/Cas9技术创制抗稻瘟病基因pi21突变体
Creation of rice blast resistant gene pi21 mutants based on CRISPR/Cas9 technology
摘要
Abstract
The rice blast resistant gene pi21 of Tai 15-7 Nuo was edited by CRISPR/Cas9 technology.Two sequences located on the first and second exons of pi21 gene were selected as targets to construct a binary expression vector pHUE411-pi21-Cas9.Twenty one T0 individual plants with Tai 15-7 Nuo as the background were obtained by Agrobacterium infection.After generation addition,6 homozygous mutants without vector were detected in 149 T,plants,including 3 types of mutations named pi21-t1,pi21-t2,pi21-t3.Each mutation resulted in the deletion of 227,239,and 99 amino acids in the protein encoded by pi21 gene.The identification results of rice blast showed that during the mature stage,only the number and area of disease spots in pi21-t3 among the 3 mutants were significantly reduced compared to Tai 15-7 Nuo,while the incidence level of the other 2 mutants was basically the same as that of Tai 15-7 Nuo.Except for improved resistance to rice blast,the mutant pi21-t3 showed no significant difference in other agronomic traits such as plant height,number of grains per spike,and seed setting rate during the mature stage compared to Tai 15-7 Nuo.This study could provide some reference for breeding rice varieties resistant to rice blast.关键词
CRISPR/Cas9技术/pi21基因/稻瘟病/纯合突变体Key words
CRISPR/Cas9 technology/pi21 gene/Rice blast/Homozygous mutant分类
农业科技引用本文复制引用
谢留杰,段敏,黄善军..基于CRISPR/Cas9技术创制抗稻瘟病基因pi21突变体[J].上海农业学报,2025,41(2):8-15,8.基金项目
浙江省自然科学基金项目(LTGN24C13002) （LTGN24C13002）
台州市农业科技计划项目(23nya09) （23nya09）
台州市农业科技计划项目(23nya18) （23nya18）
