上海农业学报2025，Vol.41Issue(2)：25-32,8.DOI:10.15955/j.issn1000-3924.2025.02.04
不同栽培配方对大球盖菇碳氮代谢相关酶活性与子实体产量的影响
Effects of different cultivation formulas on the activity of enzymes related to carbon and nitrogen metabolism and fruiting body yield of Stropharia rugosoannulata
摘要
Abstract
Using Stropharia rugosoannulata strain 'Huqiu 1' as the experimental material,this study investigated the effects of different carbon-to-nitrogen(C/N)ratios on mycelial growth,carbon-nitrogen metabolism-related enzyme activities,and yield during fruiting body development.The results demonstrated that the control group exhibited the highest glutamate synthase(GOGAT)activity and the lowest asparagine synthetase(AS)activity during both mycelial vegetative growth and fruiting body development stages,accompanied by lower yield.In the C/N 50∶1 and 60∶1 groups,β-glucosidase activity showed no significant variation;however,the C/N 60∶1 group displayed significantly higher glutamine synthetase(GS)and AS activities during the primordium formation stage compared to other C/N ratios,along with the highest fruiting body yield.The C/N 88∶1 group exhibited lower activities of GOGAT,GS,and nitrate reductase(NR).Notably,during the primordium stage,carbon metabolism-related enzymes(β-glucosidase and endo-β-1,4-glucanase)and nitrogen metabolism-related enzyme(GS)demonstrated elevated activities.In summary,the C/N 60∶1 group exhibited the highest overall activities of carbon-nitrogen metabolism-related enzymes,the earliest primordium emergence,and the maximum fruiting body yield.Therefore,the C/N ratio of 60∶1 is identified as the optimal formulation for the industrial cultivation of S.rugosoannulata.关键词
大球盖菇/碳氮比/子实体发育/碳氮代谢相关酶Key words
Stropharia rugosoannulata/Carbon to nitrogen ratio/Fruiting body development/Enzymes related to carbon and nitrogen metabolism分类
农业科技引用本文复制引用
冀浩,张津京,戴建成,郝海波,岳一鸿,陈辉..不同栽培配方对大球盖菇碳氮代谢相关酶活性与子实体产量的影响[J].上海农业学报,2025,41(2):25-32,8.基金项目
崇明区农业科创项目(2023CNKC-01-06) （2023CNKC-01-06）
