上海海洋大学学报2025，Vol.34Issue(3)：486-498,13.DOI:10.12024/jsou.20240404521
中华绒螯蟹Bco1基因的表达模式及其对高pH胁迫的响应
Expression pattern and high pH stress response of the Bco1 gene in the Chinese mitten crab(Eriocheir sinensis)
摘要
Abstract
This article combines bioinformatics,quantitative real-time PCR,in situ hybridization,and HPLC techniques to investigate the sequence characteristics,spatiotemporal expression patterns,and subcellular localization of the Bco1 gene in the Chinese mitten crab(Eriocheir sinensis).It also explores the variations in Es-Bco1 expression,color,and carotenoid content in the hepatopancreas under chronic stress caused by high pH levels.The findings reveal that the full-length of Es-Bco1 open reading frame(ORF)spans 1 614 bp and encodes 537 amino acids.It belongs to the RPE65 superfamily and exhibits a high similarity to BCO1 in the Portunus trituberculatus.(2)Es-Bco1 was expressed in multiple tissues,with high expression in the endoderm,eyestalks,and hepatopancreas;the expression of Es-Bco1 in the hepatopancreas was highest during the post-molt stage(stage AB);the expression of Es-Bco1 in the hepatopancreas significantly increased during ovarian stages Ⅰ-Ⅲ and then significantly decreased.(3)In situ hybridization results showed that Es-Bco1 mRNA was mainly localized in the F cells(Fibrillar cell)and R cells(Resorptive cell)of the hepatopancreas during the AB stage and ovarian development stage Ⅲ.(4)After 30 days of exposure to pH 8.0(control),9.0,and 10.0,the expression level of Es-Bco1 in the hepatopancreas of juvenile crabs significantly increased,while the lightness value(L*),yellowness value(b*),total carotenoid content,and β-carotene content in the hepatopancreas significantly decreased.In conclusion,Bco1 in E.sinensis is mainly expressed in the epidermal layer,eyestalks,and hepatopancreas,with high expression during the post-molt stage and ovarian stage Ⅲ.Furthermore,high pH stress can upregulate Es-Bco1 expression in the hepatopancreas and reduce carotenoid content,indicating that Bco1 plays an important role in carotenoid cleavage of E.sinensis in response to high pH stress.关键词
中华绒螯蟹/β-胡萝卜素15,15´加氧酶(BCO1)/基因表达/亚细胞定位/pH胁迫Key words
Eriocheir sinensis/β-carotene 15,15'oxygenase(BCO1)/gene expression/subcellular localization/pH stress分类
水产学引用本文复制引用
熊静怡,张敏,杨宗霖,陈晓武,姜晓东,成永旭,赵金山,吴旭干..中华绒螯蟹Bco1基因的表达模式及其对高pH胁迫的响应[J].上海海洋大学学报,2025,34(3):486-498,13.基金项目
国家自然科学基金(31873041) （31873041）
上海市科委"科技创新行动计划"启明星项目扬帆专项(23YF1416900) （23YF1416900）
山东省东营市黄河三角洲产业领军人才项目(DYRC202100215) （DYRC202100215）
上海市水产动物良种创制与绿色养殖协同创新中心项目(2021科技02-12) （2021科技02-12）
