上海海洋大学学报2025，Vol.34Issue(3)：519-529,11.DOI:10.12024/jsou.20240404473
圆紫菜快速生长新品系的分离与特性分析
Isolation and characterization of a new strain with fast growth in Pyropia suborbiculata(Bangiales,Rhodophyta)
摘要
Abstract
Compared with Pyropia haitanensis cultivated in southern China,Pyropia suborbiculata has stronger high-temperature resistance,but its round algae are not suitable for production.Therefore,several long-type mutants were isolated from the wild-type strain of P.suborbiculata by artificial mutagenesis.In this study,the blades of the long-type mutant(CCT-2)of P.suborbiculata were induced by 60Co-γ ray,and a new strain(PS-M1)with fast growth was screened from its progeny with growth rate as the main breeding index.The PS-M1 blades cultured at 23℃for 60 days had a mean length of 68.1 cm and a length-width ratio of 26.3,which were 2.7 and 2.4 times of CCT-2,respectively.The blades of PS-M1 and CCT-2 cultured at 23℃for 30 days and then cultured at 30℃for another 30 days,their mean length had increased by 6.4 and 3.0 times,respectively.The survival rates of conchospores of PS-M1 and CCT-2 were 47.4 and 42.2％,respectively,after 14 days of culture at 30℃,with no significant difference between them.The blades cultured at 23℃for 45 days had a mean chlorophyll a content of 6.5 mg/g and phycobiliprotein content of 64.9 mg/g in PS-M1,which were 20.4％and 22.9％higher than those of CCT-2,respectively.The thickness of PS-M1 was 33.6 μm,which was 15.8％thinner than that of CCT-2.The total numbers of conchospores released by PS-M1 was up to 2.223 million per shell for 10 consecutive days,which was 4.8 times that of CCT-2.In conclusion,compared with long-type mutant(CCT-2),the new strain(PS-M1)of P.suborbiculata has a higher growth rate,better resistance to high-temperature and also shows obvious advantages in main pigments protein contents,and number of conchospores releasing,which has potential to be applied in commercial cultivation.关键词
圆紫菜/60Co-γ射线/新品系/生长/耐高温Key words
Pyropia suborbiculata/60Co-γ ray/new strain/growth/high-temperature resistance分类
水产学引用本文复制引用
应家轩,丁洪昌,严兴洪..圆紫菜快速生长新品系的分离与特性分析[J].上海海洋大学学报,2025,34(3):519-529,11.基金项目
国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项(2023YFD2400103,2023YFD2400102) （2023YFD2400103,2023YFD2400102）
国家自然科学基金青年基金(32102773) （32102773）
上海市青年科技英才扬帆计划(21YF1417100) （21YF1417100）
上海海洋大学青年教师科研启动项目(A2-2006-23-200304) （A2-2006-23-200304）
