山西中医药大学学报2025，Vol.26Issue(3)：282-287,293,7.DOI:10.19763/j.cnki.2096-7403.2025.03.09
基于淋巴细胞亚群和细胞因子筛选区别原发性干燥综合征燥湿互结证和气阴两虚证的标志物
Markers screening for differentiating primary Sjögren's syndrome with the syndrome of intermingled dryness and dampness and syndrome of deficiency of both Qi and Yin based on lymphocyte subsets and cytokines
摘要
Abstract
Objective:The syndrome of intermingled dryness and dampness and syndrome of deficiency of both Qi and Yin are common traditional Chinese medicine(TCM)syndromes in primary Sjögren's syndrome(pSS).However,differentiat-ing these two syndromes from a TCM perspective is challenging,which limits the improvement and promotion of TCM efficacy.This study aims to screen biomarkers for differentiating the syndrome of intermingled dryness and dampness and syndrome of deficiency of both Qi and Yin in pSS from the perspective of lymphocyte subsets and cytokines.Methods:Patients hospital-ized in the Rheumatology and Immunology Department of the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from June 2020 to September 2023,diagnosed with pSS with the syndrome of intermingled dryness and dampness(35 cases),syndrome of dampness-heat accumulation and obstruction(8 cases),and syndrome of deficiency of both Qi and Yin(61 cases),were included in the study.Retrospective analysis was conducted to compare different TCM syn-dromes in terms of CD4+T cell subsets,CD8+T cell subsets,NK cells,B lymphocytes,Th1,Th2,and Th17 cytokines,as well as erythrocyte sedimentation rate(ESR),C-reactive protein(CRP),immunoglobulins,complements,and autoantibod-ies.Results:In terms of lymphocyte subsets,patients with the syndrome of intermingled dryness and dampness had signifi-cantly higher counts of CD3+T cells,CD4+T cells,and CD16+56+NK cells compared to those with the syndrome of deficiency of both Qi and Yin,while the percentage of CD8+T cells was significantly lower,and the CD4+/CD8+T cell ratio was signifi-cantly higher.In terms of cytokines,patients with the syndrome of intermingled dryness and dampness had significantly higher levels of IL-6 and IL-17 compared to those with the syndrome of deficiency of both Qi and Yin.There were no significant dif-ferences in the levels of other cytokines between the two groups(P>0.05).ROC curve analysis showed that the CD4+/CD8+T cell ratio,and the levels of IL-6 and IL-17 could differentiate the syndrome of intermingled dryness and dampness from the syndrome of deficiency of both Qi and Yin,with area under the curve(AUC)values of 0.63,0.678 and 0.622,respectively.Conclusion:The syndrome of intermingled dryness and dampness significantly differs from the syndrome of deficiency of both Qi and Yin in terms of CD4+T cell count,CD16+56+NK cell count,CD4+/CD8+T cell ratio,CD8+T cell percentage,and levels of IL-6 and IL-17.These differences suggest that the syndrome of intermingled dryness and dampness may have a stronger immune activation state and inflammatory response.Particularly,changes in the CD4+/CD8+T cell ratio and levels of IL-6 and IL-17 are helpful for differentiating the syndrome of intermingled dryness and dampness from the syndrome of defi-ciency of both Qi and Yin,providing a biological basis for TCM syndrome differentiation and treatment.关键词
原发性干燥综合征/燥湿互结证/气阴两虚证/淋巴细胞亚群/细胞因子Key words
pSS/syndrome of intermingled dryness and dampness/syndrome of deficiency of both Qi and Yin/lym-phocyte subsets/cytokines分类
医药卫生引用本文复制引用
张雨晴,黄新福,侯雷,马武开,杨鹏..基于淋巴细胞亚群和细胞因子筛选区别原发性干燥综合征燥湿互结证和气阴两虚证的标志物[J].山西中医药大学学报,2025,26(3):282-287,293,7.基金项目
贵州省高等学校中西医结合防治疾病转化医学重点实验室(黔教技[2023]017号) （黔教技[2023]017号）
