上海针灸杂志2025，Vol.44Issue(5)：605-612,8.DOI:10.13460/j.issn.1005-0957.2024.13.4034
电针对炎性痛小鼠前扣带回皮层N-甲基-D-天冬氨酸受体2B亚基和磷酸酶2A表达的影响
Effect of electroacupuncture on the expression of NR2B and PP2A in the anterior cingulate cortex of inflammatory pain mice
摘要
Abstract
Objective To observe the effects of electroacupuncture(EA)on the behaviors of mice with complete Freund's adjuvant(CFA)-induced inflammatory pain and the expression of N-methyl-D-aspartate receptor subunit 2B(NR2B)and protein phosphatase 2A(PP2A)in the anterior cingulate cortex(ACC),and to discuss the role of NR2B and PP2A in the analgesic effect of EA.Method Twenty-four male C57BL/6 mice were randomly allocated to a control group,a model group,and an EA group,each consisting of 8 mice.Mice in the model group received an injection of 25 μL of CFA at the right hind paw to establish inflammatory pain models.Mice in the EA group received EA at Zusanli(ST36)and Sanyinjiao(SP6)on the right side at post-modeling day 1,day 3,day 5,and day 7.The mechanical paw withdrawal rate and thermal paw withdrawal latency of both hind paws were observed in the three groups of mice.The protein expression of NR2B and PP2A in the ACC was detected using the Western blot(WB),immunohistochemistry(IHC),and immunofluorescence(IF)assays.Result Compared to the control group,the mechanical paw withdrawal rate increased notably(P＜0.05)and the thermal paw withdrawal latency dropped significantly(P＜0.05)in the model group at post-modeling day 1,day 3,day 5,and day 7.Compared to the model group,the mechanical paw withdrawal rate decreased notably(P＜0.05)and the thermal paw withdrawal latency increased markedly(P＜0.05)in the EA group at post-modeling day 1,day 3,day 5,and day 7.The WB results demonstrated that the model group had decreased PP2A protein expression(P＜0.05)and increased NR2B protein expression(P＜0.05)in the ACC compared to the control group;compared to the model group,the expression of PP2A increased notably(P＜0.05)and the expression of NR2B dropped significantly(P＜0.05)in the EA group.The IHC findings showed that compared to the control group,the model group had notably decreased PP2A expression in the ACC(P＜0.05),and the EA group had increased PP2A expression compared to the model group(P＜0.05).The IF assay revealed that compared to the control group,the model group had significantly increased NR2B protein expression in the ACC(P＜0.05),and the EA group had decreased NR2B protein expression compared to the model group(P＜0.05).Conclusion EA can improve hyperalgesia in inflammatory pain mice by down-regulating the expression of NR2B and up-regulating the expression of PP2A in the ACC,indicating that NR2B and PP2A may play a role in EA's effects in reducing inflammatory pain.关键词
电针/疼痛/针刺镇痛/N-甲基-D-天冬氨酸受体2B亚基/磷酸酶2A/小鼠Key words
Electroacupuncture/Inflammatory pain/Acupuncture analgesia/N-methyl-D-aspartate receptor subunit 2B/Protein phosphatase 2A/Mice分类
医药卫生引用本文复制引用
顾益枭,陈敏娟,陈潇男,陈玲阳..电针对炎性痛小鼠前扣带回皮层N-甲基-D-天冬氨酸受体2B亚基和磷酸酶2A表达的影响[J].上海针灸杂志,2025,44(5):605-612,8.基金项目
浙江省台州市科技计划项目(22ywb18) （22ywb18）
浙江省医药卫生项目(2023RC301) （2023RC301）
