世界中西医结合杂志2025，Vol.20Issue(4)：637-644,8.DOI:10.13935/j.cnki.sjzx.250401
补肾化瘀生新方防治心肌梗死后心室重构的分子机制研究
Molecular Mechanisms of Bushen Huayu Shengxin Prescription in the Prevention and Treatment of Ventricular Remodeling after Myocardial Infarction
摘要
Abstract
Objective To explore the molecular mechanism of Bushen Huayu Shengxin prescription in preventing and treating ventricular remodeling after myocardial infarction.Methods Traditional Chinese Medicine Systems Pharma-cology(TCMSP)and UniProt database were utilized to obtain the compounds and target genes of Bushen Huayu Shengxin prescription.The GeneCards database was employed to screen for target genes associated with ventricular remodeling after myocardial infarction.The STRING database was used to construct a protein-protein interaction(PPI)network,as well as gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Then,R software was a-dopted to get intersecting targets.Autodock Tools software resorted to molecular docking between potential targets and blood components.The Olivette method was taken to establish an acute myocardial infarction rat model.After 14 days of continu-ous gavage administration,materials were taken and serum samples were collected.Liquid chromatography-tandem mass spectrometry(LC-MS/MS)was used for analyzing drug components.The quantitative real-time polymerase chain reac-tion(qRT-PCR)was used to verify core targets and key signaling pathways that exhibit strong correlations.Results The microarray analysis revealed that the primary bioactive components of Bushen Huayu Shengxin prescription detected in the blood were catalpol,rehmapicroside,rubiadin,physcion,baicalin,and ferulic acid.A total of 3878 differential genes were i-dentified in myocardial infarction mRNA samples,with 47 target genes associated with the Bushen Huayu Shengxin pre-scription.Protein-protein interaction(PPI)network analysis indicated that the core targets included TLR4,NLRP3,Caspase-1,GSDMD,NF-κB,and IL-1 β.KEGG pathway enrichment analysis identified 107 signaling pathways,inclu-ding PI3K/Akt,HIF-1,and NF-κB/NLRP3.Compared to the normal group,the mRNA expression levels of TLR4,NL-RP3,Caspase-1,GSDMD,NF-κB,and IL-1 β in the heart tissue of the model group were significantly elevated.Treat-ment with Bushen Huayu Shengxin prescription significantly reduced the expression of these genes in the heart tissue of the model group(P＜0.05).Conclusion Bushen Huayu Shengxin prescription regulates the NF-κB/NLRP3 signaling path-way,as well as its upstream and downstream signaling factors through the ciRS-7/miR-7 axis,intervenes in the inflam-matory response and cell pyroptosis after myocardial infarction and reduces the incidence of ventricular remodeling after myocardial infarction.关键词
骨髓间充质干细胞源外泌体/ciRS-7/miR-7轴/NF-κB/NLRP3信号通路/补肾化瘀生新方/心室重构/心肌梗死Key words
Exosomes Derived from Bone Marrow Mesenchymal Stem Cell(BMSCs-Exo)/ciRS-7/miR-7 Ax-is/NF-κB/NLRP3 Signaling Pathway/Bushen Huayu Shengxin Prescription/Ventricular Remodeling/Myocardial Infarc-tion分类
医药卫生引用本文复制引用
张金生,安兰花,解冬,杨联河,王绍娜,曹文君,惠小珊,寇金康..补肾化瘀生新方防治心肌梗死后心室重构的分子机制研究[J].世界中西医结合杂志,2025,20(4):637-644,8.基金项目
河南省高等学校重点科研项目基础研究项目(24A360016) （24A360016）
河南省中医药科学研究专项课题(2022ZY1110、2019JDZX2095) （2022ZY1110、2019JDZX2095）
