饲料研究2025，Vol.48Issue(7)：62-67,6.DOI:10.13557/j.cnki.issn1002-2813.2025.07.012
乙醇梭菌蛋白替代鱼粉对育成期乌苏里貉生长性能及血清生化指标的影响
Effect of Clostridium autoethanogenum protein replacing fish meal on growth performance and serum biochemical indexes of Ussuri raccoon dogs during breeding period
摘要
Abstract
The study aimed to investigate the effects of different levels of Clostridium autoethanogenum protein as a substitute for fish meal on the growth performance and serum biochemical indexes of male Ussuri raccoon dogs during the breeding period.A single-factor experimental design was employed,involving 60 healthy male Ussuri raccoon dogs(130 days old)with similar body weights.The Ussuri raccoon dogs were randomly divided into four groups,with 15 replicates per group and one Ussuri raccoon dogs per replicate.The control group(group Ⅰ)was fed a basal diet without Clostridium autoethanogenum protein,while the experimental groups were fed diets supplemented with 0.5%(group Ⅱ:replacing 10%fish meal),1.0%(group Ⅲ:replacing 20%fish meal),and 1.5%(group Ⅳ:replacing 30%fish meal)Clostridium autoethanogenum protein.The pre-experimental period lasted seven days,followed by a 40-day formal experimental period.The results showed that there were no significant differences in the average body weight,serum total protein(TP),albumin(ALB),cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),aspartate aminotransferase(AST)activity and total antioxidant capacity(T-AOC)among the groups(P>0.05).Compared with group Ⅰ,the average daily gain of Ussuri raccoon dogs in group Ⅱ was significantly increased(P<0.05).Compared with group Ⅰ,the serum alanine aminotransferase(ALT)activity in group Ⅳ was significantly increased(P<0.05),and the serum urea nitrogen(BUN)content in group Ⅱ was significantly decreased(P<0.05).The serum activity of alkaline phosphatase(ALP)was significantly increased in group Ⅲ(P<0.05),and the concentrations of triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)were significantly decreased(P<0.05).Compared with group Ⅰ,the serum concentrations of immunoglobulin G(IgG),immunoglobulin A(IgA),immunoglobulin M(IgM),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in the other groups were significantly increased(P<0.05).Compared with group Ⅰ,the serum malondialdehyde(MDA)content in group Ⅲ and group Ⅳ was significantly reduced(P<0.05).The activity of superoxide dismutase(SOD)in serum was significantly increased in the Ⅳ group(P<0.05).The results show that the use of Clostridium autoethanogenum protein to replace 30%of the fish meal in the diet(with an addition rate of 1.5%)has no significant effect on the growth performance of Ussuri raccoon dogs,and the economic benefit of replacing 10%fish meal(with an addition of 0.5%)was the best,and the growth performance and immune performance of Ussuri raccoon dogs can be improved.关键词
乌苏里貉/育成期/乙醇梭菌蛋白/鱼粉/生长性能/血清生化指标Key words
Ussuri raccoon dogs/breeding period/Clostridium autoethanogenum protein/fish meal/growth performance/serum biochemical indexes分类
农业科技引用本文复制引用
李重阳,李孟韩,邱凯,马世峰,耿仕夏,王静,姚婷,张海华..乙醇梭菌蛋白替代鱼粉对育成期乌苏里貉生长性能及血清生化指标的影响[J].饲料研究,2025,48(7):62-67,6.基金项目
国家重点研发计划项目(项目编号:2023YFD1302000) （项目编号:2023YFD1302000）
河北省重点研发计划项目(项目编号:223226613D) （项目编号:223226613D）
