饲料研究2025,Vol.48Issue(7):73-78,6.DOI:10.13557/j.cnki.issn1002-2813.2025.07.014
复合酵母菌发酵产δ-氨基乙酰丙酸的研究
Study on production of δ-aminolevulinic acid by fermentation of complex yeast
摘要
Abstract
The research aims to improve the production of δ-aminolevulinic acid(δ-ALA)in yeast culture by compounding strains and optimizing cultivation conditions.The experiment selected a compound of Candida lipolytica and Saccharomyces cerevisiae FQ17,and optimized the culture medium and conditions through single factor and orthogonal experiments.The production of δ-ALA in yeast culture was determined by spectrophotometry.The results showed that according to single factor and orthogonal experiment,the optimal culture conditions were as follows:Strain ratio 1∶2,inoculation amount 8%,molasses amount 4.75%,urea amount 0.050%,glucose amount 2.25%,glycine amount 20%,zinc sulfate amount 0.055%,magnesium sulfate amount 0.100%,iron sulfate amount 0.055%and chlorine sodium content 0.005%,the yield of δ-ALA in the yeast culture could reach 471.40 mg/kg.The research results indicate that composite yeast can significantly increase the production of δ-ALA in yeast culture.关键词
酵母培养物/δ-氨基乙酰丙酸/菌种复配/培养条件Key words
yeast culture/δ-aminolevulinic acid/compounding strains/cultivation condition分类
农业科技引用本文复制引用
林子滢,任文彬,辛璇,杨小慧,汪薇..复合酵母菌发酵产δ-氨基乙酰丙酸的研究[J].饲料研究,2025,48(7):73-78,6.基金项目
广州市科技计划项目(项目编号:2023E04J1228) (项目编号:2023E04J1228)
梅州市科技计划项目(项目编号:2023A0304057) (项目编号:2023A0304057)