饲料研究2025，Vol.48Issue(7)：79-84,6.DOI:10.13557/j.cnki.issn1002-2813.2025.07.015
基于代谢组学研究野生型五倍子和培育型五倍子差异代谢物分析
Metabolomics analysis of differential metabolites between wild gallnuts and cultivated gallnuts
摘要
Abstract
The aim of this study was to elucidate the distribution of main characteristic metabolites in four kinds of gallnut(wild-type horned gallnut,belly gallnut and cultivated horned gallnut,belly gallnut)based on metabolomics.After the test was detected by LC-MS/MS system,the online METLIN database in Progenesis QI software,public database and Baimaik self-built database were used for identification,and the differential metabolites of different gallnut were analyzed.The results showed that a total of 6 347 metabolites were annotated in the experiment.The total chemical components of wild-type horned gallnut and belly gallnut were similar,the tannin and phenolic compositions of wild-type horned gallnut and cultivated horned gallnut showed high similarity,as do those of wild-type belly gallnut and cultivated belly gallnut.The tannins in gallnut are gallic acid mixtures,including gallic acid,hydrated gallic acid,tannic acid,trimeric gallic acid and gallic acid-4-O-glucoside.The most abundant phenols in the four gallnuts were resorcinol,pyrogallol,3,4-dihydroxymandelate,2,4-dinitrophenol,and lactucin.The different phenolic metabolites in wild-type horned gallnut,belly gallnut and cultivated horned gallnut included limonone,2,3-dihydroxytoluene,3,4-dihydroxyphenylacetic acid,tyrosol and 4-n-nonylphenol.There were significant differences in coniferaldehyde and 5-hydroxyconiferol between cultivated belly gallnut and wild-type belly gallnut.The study indicates that the experiment can provide reference for the application of gallnut in medicine,animal disease treatment,feed industry development and so on.关键词
五倍子/代谢组学/角倍/肚倍/分布规律Key words
gallnut/metabonomics/horned gallnut/belly gallnut/distribution patterns分类
农业科技引用本文复制引用
李志惠,张富林,管丽飞,熊伟,李任军..基于代谢组学研究野生型五倍子和培育型五倍子差异代谢物分析[J].饲料研究,2025,48(7):79-84,6.基金项目
贵州省科技计划项目(项目编号:黔科合支撑[2023]一般470) （项目编号:黔科合支撑[2023]一般470）
贵州省科技计划项目(项目编号:黔科合成果[2021]一般040) （项目编号:黔科合成果[2021]一般040）
