饲料研究2025，Vol.48Issue(7)：96-101,6.DOI:10.13557/j.cnki.issn1002-2813.2025.07.018
低共熔溶剂提取桉叶多酚工艺优化及其抗氧化活性研究
Optimization of polyphenol extraction from eucalyptus leaves with deep eutectic solvents and study on its antioxidant activity
摘要
Abstract
This study optimized the extraction process of polyphenols from eucalyptus leaves using deep eutectic solvents with choline chloride and betaine as hydrogen bond acceptors through single-factor and orthogonal experiments,and evaluated their antioxidant activity.The results showed that the optimal conditions were as follows:A betaine-to-ethylene glycol ratio of 2∶1,water content of 50%,liquid-to-material ratio of 40 mL/g,and ultrasonic power of 300 W,ultrasonic temperature of 75℃,ultrasonic time of 50 min.Under these conditions,the extraction yield of eucalyptus polyphenols reached 22.39%,significantly higher than that of traditional solvents.The eucalyptus leaf extract exhibited stronger scavenging effects on 2,2-diazo-di(3-ethyl-benzothiazolin-6-sulfonic acid)diamiammonium salt(ABTS+)radicals compared to 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and nitrogen dioxide radicals,with maximum scavenging rates of 98.8%,83.5%,and 77.5%,respectively.The corresponding IC50 values were 4.41,4.82,and 324.70 mg/L.The study demonstrates that the betaine/ethylene glycol-based deep eutectic solvents system achieves high extraction efficiency and excellent antioxidant activity for eucalyptus polyphenols,providing a reference for the future development and utilization of eucalyptus resources.关键词
低共熔溶剂/桉叶/多酚/抗氧化活性/工艺优化Key words
deep eutectic solvents/eucalyptus leaves/polyphenols/antioxidant activity/process optimization分类
农业科技引用本文复制引用
蒋利荣,曾湘楚,张高荣,汪艳平,李金月,陈娟林,黄秀香..低共熔溶剂提取桉叶多酚工艺优化及其抗氧化活性研究[J].饲料研究,2025,48(7):96-101,6.基金项目
广西自然科学基金项目(项目编号:2020GXNSFAA297218) （项目编号:2020GXNSFAA297218）
广西现代蚕桑丝绸协同创新中心立项课题(项目编号:2022GXCSSC05、2023GXCSSC8) （项目编号:2022GXCSSC05、2023GXCSSC8）
河池学院2024年课程思政示范课程项目(项目编号:2024kcsz12) （项目编号:2024kcsz12）
广西高校中青年教师基础能力提升项目(项目编号:KY2016LX281、2022KY0611) （项目编号:KY2016LX281、2022KY0611）
