饲料研究2025，Vol.48Issue(7)：108-114,7.DOI:10.13557/j.cnki.issn1002-2813.2025.07.020
响应面法优化木棉花多糖的提取工艺及其体外抗氧化活性评估
Optimization of extraction process of Bombax malabaricum DC.polysaccharide by response surface method and evaluation of its antioxidant activity in vitro
黄璇莹 1罗锦霞 1林钦恒 1李维嘉 1黄启红 1伍玲燕1
作者信息
- 1. 广东省科学院测试分析研究所(中国广州分析测试中心)广东省化学测量与应急检测技术重点实验室 广东省保健食品功效成分检测与风险物质快速筛查工程技术研究中心,广东 广州 510000
- 折叠
摘要
Abstract
The study aimed to optimize the microwave-assisted deep eutectic solvent(DES)extraction process of Bombax malabaricum DC.polysaccharides and evaluate the antioxidant activity of the polysaccharides before and after purification.The DES composed of choline chloride and formic acid at a molar ratio of 1∶1 was selected as the extraction solvent.Based on single-factor experiments,the water content of DES,liquid-to-solid ratio,microwave temperature,and extraction time were chosen as independent variables,while the extraction yield of Bombax malabaricum DC.polysaccharides was the dependent variable,response surface methodology was employed to optimize the extraction process.Using vitamin C(VC)as a positive control,the scavenging abilities of Bombax malabaricum DC.polysaccharides before and after purification against 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals and hydroxyl radicals were evaluated.The results showed that the optimal extraction conditions were:DES water content of 42%,liquid-to-solid ratio of 40 mL/g,microwave temperature of 108℃,and extraction time of 21 min.Under these conditions,the extraction yield of Bombax malabaricum DC.polysaccharides reached 174.9 mg/g.The antioxidant activity tests showed that the scavenging ability of Bombax malabaricum DC.polysaccharide on DPPH and hydroxyl free radical increased with the increase of mass concentration of 0.1～3.0 g/L,and the scavenging ability of purified Bombax malabaricum DC.polysaccharides on DPPH and hydroxyl free radical was significantly stronger than that of Bombax malabaricum DC.polysaccharides.The study indicates that microwave-assisted DES extraction of Bombax malabaricum DC.polysaccharides is green and efficient,providing a reference for its application in feed research.关键词
木棉花多糖/微波辅助提取/低共熔溶剂/响应面法/抗氧化活性Key words
Bombax malabaricum DC.polysaccharides/microwave-assisted extraction/deep eutectic solvent/response surface methodology/antioxidant activity分类
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黄璇莹,罗锦霞,林钦恒,李维嘉,黄启红,伍玲燕..响应面法优化木棉花多糖的提取工艺及其体外抗氧化活性评估[J].饲料研究,2025,48(7):108-114,7.
