水生态学杂志2025，Vol.46Issue(3)：13-21,9.DOI:10.15928/j.1674-3075.202412050002
TBP与LEV混合物对铜绿微囊藻和斜生栅藻的联合毒性效应
Combined Toxicity of Tributyl Phosphate and Levofloxacin on Microcystis aeruginosa and Scenedesmus obliquus
摘要
Abstract
Organophosphate flame retardants(OPFRs)are widely used in industrial materials to reduce fire risk and as stabilizers.Aquatic ecosystems are the primary sink of OPFRs,which often coexist with antibiotics and other pollutants in the water environment.However,studies on the combined toxicity of OPFRs and antibiotics to microscopic primary producers are lacking.In this study,Microcystis aeruginosa(a common cyanobacteria)and Scenedesmus obliquus(a common green algae)were selected as test organ-isms,and we explored individual and combined toxicities of Tributyl phosphate(TBP),a typical OPFR,and levofloxacin(LEV),a typical antibiotic,on the two test organisms.The individual toxicities of TBP and LEV were tested using the single toxicity test method with 12 concentration treatments and the direct equipartition ray method to test their combined toxicity,with TBP to LEV concentration ratios of 1:5,2:4,3:3,4:2 and 5:1.The independent action model(IA model)was employed to discern the mode of joint tox-ic action and evaluate its severity,with a focus on exploring the response of simultaneous exposure to TBP and LEV.The LC50 of TBP toward M.aeruginosa and S.obliquus were 2.0×10-6 and 6.7×10-5 mol/L,respectively.The LC50 of LEV against M.aeruginosa and S.obliquus were 3.4×10-7 and 4.3×10-6 mol/L,respectively.Thus,M.aeruginosa is more sensitive to TBP and LEV than S.obliquus.The results of the combined exposure based on an equipartition ray design shows that the combined toxicity intensity de-creased with decreasing LEV concentration,and the toxic effect on M.aeruginosa was higher than that of S.obliquus.In addition,the toxic mode of the combined exposure to both test organisms was concentra-tion-dependent,showing synergism at low concentration and antagonism at high concentration,and the toxic intensity varied with the ratio of TBP to LEV concentration.There was a positive correlation be-tween the proportion of LEV and the toxicity of the mixture,and a negative correlation between the pro-portion of TBP and toxicity of the mixture.Both individually and combined,TBP and LEV exhibited hor-mesis on algal growth,i.e.,low concentrations promoted growth while high concentrations inhibited growth.The results of this study provide data support for evaluating the toxicity of organophosphorus flame retardants and antibiotics to aquatic organisms and their environmental risks.关键词
磷酸三丁酯/左氧氟沙星/铜绿微囊藻/斜生栅藻/联合毒性/毒物兴奋效应Key words
tributyl phosphate/levofloxacin/Microcystis aeruginosa/Scenedesmus obliquus/combined toxicity/hormesis分类
医药卫生引用本文复制引用
沈洪艳,刘爱真,杨雷,孙新宇,白玉玮,宁静,孙昊宇..TBP与LEV混合物对铜绿微囊藻和斜生栅藻的联合毒性效应[J].水生态学杂志,2025,46(3):13-21,9.基金项目
国家自然科学基金(42277270,22006116) （42277270,22006116）
河北省化工行业挥发性有机物检测与治理技术创新中心基金(ZXJJ20240102) （ZXJJ20240102）
石家庄高新区给水管网水力计算模型研究项目(XQ2024-01). （XQ2024-01）
