水生态学杂志2025，Vol.46Issue(3)：50-60,11.DOI:10.15928/j.1674-3075.202412300001
纳米二氧化钛影响泥蚶受精的作用机理
Effect of Nano Titanium Dioxide on Fertilization of Tegillarca granosa
摘要
Abstract
In this study,we investigated the effects of environmental nano titanium dioxide(nTiO2)expo-sure on sperm motility and fertilization of the marine bivalve Tegillarca granosa(blood clam)with broad-cast fertilization.The mechanism was closely examined in terms of sperm motility,energetics,intracellu-lar Ca2+homeostasis and oxidative stress.The concentration range of existing environmental nTiO2 was obtained from previous reports and T.granosa sperm was exposed to four TiO2 treatments for two hours:a control group,a bulk TiO2 group at100 μg/L,and two nano TiO2 groups at 10 μg/L nTiO2 and 100 μg/L nTiO2.The high nTiO2 exposure significantly(P<0.05)reduced the fertilization rate of T.granosa,by 20.94%compared to the control group,but the low nTiO2 and bulk TiO2 exposures did not significantly affect the fertilization rate.After exposure to 10 μg/L and 100 μg/L of nTiO2,the curvilinear velocity(VCL)and the straight line velocity(VSL)of T.Granosa sperm both significantly decreased:the VCL by 16.03%and 29.04%and the VSL by 20.21%and 63.99%,but the effects were not significant in the bulk TiO2 treatment group.While the high nTiO2 exposure significantly(P<0.05)decreased the average path velocity(VAP)of T.Granosa sperms by 31.75%,and the low nTiO2 and bulk TiO2 treatment had no obvi-ous effect on the VAP.Thus,high concentration of nTiO2 significantly impaired sperm swimming speed.Moreover,nTiO2 exposure significantly inhibited the activities of pyruvate kinase(PK),phosphofructoki-nase(PFK),and Ca2+-ATPase in the sperm,which decreased levels of ATP and Ca2+.Results also show that nTiO2 exposure markedly increased the levels of reactive oxygen species(ROS)and malondialdehyde(MDA).In conclusion,our results indicate that nTiO2 can impair the sperm motility and fertilization of T.granosa by limiting energy supply,interfering with intracellular Ca2+regulation,and inducing oxidative stress.关键词
纳米二氧化钛/泥蚶/受精/精子运动Key words
nano titanium dioxide(nTiO2)/Tegillarca granosa/fertilization/sperm motility分类
生物科学引用本文复制引用
俞行洲,旃梓严,漆金宵,刘广绪,赵信国,韩毓..纳米二氧化钛影响泥蚶受精的作用机理[J].水生态学杂志,2025,46(3):50-60,11.基金项目
国家自然科学基金面上项目"抗抑郁药氟西汀对泥蚶受精的影响及其作用机理研究"(NO.32172944) （NO.32172944）
国家自然科学基金青年项目,捕食压力下海洋酸化对紫贻贝群聚习性的影响(42106195). （42106195）
