水土保持研究2025，Vol.32Issue(4)：33-40,49,9.DOI:10.13869/j.cnki.rswc.2025.04.010
堆肥污泥添加对黄土丘陵区草地土壤酶活性及动力学特征的影响
Effects of compost sludge addition on soil enzyme activities and kinetic characteristics in grassland soil of the Loess hilly region
摘要
Abstract
[Objective]This study aims to uncover the dominant driving factors of soil enzyme activity characteristics,providing a scientific basis for the resource utilization of compost sludge and the improvement of soil quality.[Methods]An indoor incubation experiment was conducted to investigate the effects of composted sludge application rates(0%,2%,5%,10%,15%and 20%)and incubation temperatures(5℃,15℃,25℃and 35℃)on the activities of β-glucosidase(BG),N-acetyl-β-D-glucosaminidase(NAG),and alkaline phosphatase(ALP)as well as their kinetic parameters in typical grassland soils of the Loess hilly region.[Results]The results showed that the highest activities of BG and ALP were observed with 5%compost sludge addition,while NAG activity peaked at 15%compost sludge addition.The soil enzyme activities at 35℃were 17%～168%higher compared to 5℃.The maximum reaction rate(Vmax)of BG and ALP increased by 27%～73%and 68%～151%,respectively,with 5%composted sludge addition compared to the control,while the Vmax of NAG increased by 82%～289%with 15%composted sludge addition.The catalytic efficiency(Kcat)of BG reached its peak with 10%composted sludge addition,increasing by 46%～150%compared to the control.Meanwhile,the Kcat of NAG and ALP increased by 335%～589%and 3%～51%,respectively,with 15%composted sludge addition.Redundancy analysis showed that nitrate nitrogen,total phosphorus,organic carbon,and available phosphorus were the main factors affecting soil enzyme activities and kinetic parameters.[Conclusion]Applying moderate amounts(5%～15%)of composted sludge in degraded grasslands of the Loess hilly region significantly enhanced soil enzyme activities and kinetic parameters,greatly improving soil quality.In particular,5%compost sludge significantly increased BG and ALP activities,whereas 15%compost sludge significantly enhanced NAG activity,highlighting the positive role of compost sludge in soil restoration.关键词
堆肥污泥/酶活性/动力学参数Key words
composted sludge/soil enzyme activity/soil enzyme kinetic parameters分类
农业科学引用本文复制引用
艾祥宇,肖列,李鹏,耿直,王周,马园园..堆肥污泥添加对黄土丘陵区草地土壤酶活性及动力学特征的影响[J].水土保持研究,2025,32(4):33-40,49,9.基金项目
国家自然科学基金(42377350,42330719) （42377350,42330719）
陕西省教育厅协同创新中心项目(22JY043) （22JY043）
