生物安全学报（中英文）2025，Vol.34Issue(2)：114-123,10.DOI:10.3969/j.issn.2095-1787.20240121
福寿螺肠道微生物群落结构与功能的性别差异
Sex differences in the structure and function of gut microbiota in Pomacea canaliculata
摘要
Abstract
[Aim]This study explores the sex differences in the structure and function of gut microbiota in Pomacea canaliculata,aiming to provide a scientific basis for analyzing the sex differences in the environmental adaptability of P.canaliculata during the in-vasion process.[Method]High-throughput sequencing was employed to analyze the gut samples of male and female snails from dif-ferent geographical locations(Hainan,Guangdong,and Fujian).The diversity indices of gut microbiota were comprehensively ana-lyzed.Non-metric multidimensional scaling(NMDS),permutational multivariate analysis of variance(PERMANOVA),and Tax4Fun functional prediction were conducted to analyze the composition,abundance differences,structural similarities,and func-tional differences of gut microbiota in male and female snails.[Result]The Chao1 index of gut microbiota showed sex differences(P＜0.05),which were not observed in other alpha diversity indices(P＞0.05).Sex differences(P＜0.05)existed in the abundance of some phyla(Firmicutes,Bacteroidota,Spirochaetota,and Cyanobacteria)and a genus(Lactococcus)of the gut microbiota.The ge-ographical location significantly affected the β diversity of gut microbiota in P.canaliculata,while sex had no significant effect on it.In addition,there was no significant difference in the abundance of KEGG pathways in the gut microbiota of male and female snails from Hainan.Male snails had higher abundance of membrane transport pathways than female snails from Guangdong(P＜0.05).A-mong the samples from Fujian,male snails had higher abundance of cellular processes and signaling pathways(P=0.01),and fe-male snails had higher abundance of translation pathways(P＜0.05).[Conclusion]Sex mainly changes the abundance rather than diversity of gut microbiota,while geographical location significantly shapes the community structure and function of gut microbiota in P.canaliculata.关键词
福寿螺/肠道微生物/群落特征/性别差异Key words
Pomacea canaliculata/gut microbiota/community characteristics/sex differences引用本文复制引用
黄哲,罗思,房苗,徐猛,余梵冬,舒璐,韦慧,汪学杰,金诗语,顾党恩..福寿螺肠道微生物群落结构与功能的性别差异[J].生物安全学报（中英文）,2025,34(2):114-123,10.基金项目
广州市科技计划项目(2023B03J1306) （2023B03J1306）
中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2024SJRC12) （2024SJRC12）
国家大宗淡水鱼产业技术体系(CARS-45) （CARS-45）
广东省现代农业产业共性关键技术研发创新团队建设项目(2023KJ134) （2023KJ134）
中国水产科学研究院基本科研业务费(2023TD17) （2023TD17）
