生物安全学报（中英文）2025，Vol.34Issue(2)：130-136,7.DOI:10.3969/j.issn.2095-1787.20240022
转基因抗虫玉米浙大瑞丰8实时荧光定量PCR检测方法
Real-time fluorescent quantitative PCR for detection of transgenic insect-resistant maize Zheda Ruifeng 8
摘要
Abstract
[Aim]Genetically modified(GM)insect-resistant maize,Zheda Ruifeng 8,is a new type of insect-resistant maize developed by Hangzhou Ruifeng Biotechnology Co.Ltd.,and it was approved for an agricultural genetically modified organism safety certificate(for production and application)in 2021.This study aimed to design specific amplification primers and TaqMan probes for the transgenic insect-resistant maize Zheda Ruifeng 8 and to quantitatively detect its transgenic components,thereby providing a basis for safety supervision by regulatory authorities.[Method]Through a series of experiments,including system optimization,accuracy and precision testing,and robustness testing,specific primers and probes were designed to quantitatively detect the transgenic components of Zheda Ruifeng 8.[Result]The data showed that the method can specifically and quantitatively detect the transgenic components of Zheda Ruifeng 8.Positive samples with a copy number ratio of＞0.1％(20 copies)were accurately quantified,and positive samples with a copy number ratio of 0.05％(ten copies)were specifically detected.[Conclusion]The findings of this study provide effective support for safety supervision by regulatory authorities,thereby indicating that the developed method can be used for the quantitative detection and safety supervision of Zheda Ruifeng 8.关键词
转基因抗虫玉米浙大瑞丰8/实时荧光定量PCR/检测Key words
transgenic insect-resistant maize Zheda Ruifeng 8/real-time fluorescence quantitative PCR/detection引用本文复制引用
田锦,梁晋刚,句荣辉,蒋红叶,陈子言,张华,李凌燕,孙宇,王颢潜,陈红..转基因抗虫玉米浙大瑞丰8实时荧光定量PCR检测方法[J].生物安全学报（中英文）,2025,34(2):130-136,7.基金项目
农业农村部农业国家和行业标准制修订项目(农质标函[2022]66号) （农质标函[2022]66号）
科技创新2030重大项目(2022ZD0401909) （2022ZD0401909）
农业科研杰出人才培养计划 （）
