生物安全学报（中英文）2025，Vol.34Issue(2)：145-154,10.DOI:10.3969/j.issn.2095-1787.20230153
杀螺胺乙醇胺盐和四聚乙醛不同致死浓度对福寿螺肠道微生物群落特征的影响
Effects of different lethal concentrations of niclosamide ethanolamine salt and metaldehyde on the gut microbiota structure of Pomacea canaliculata
摘要
Abstract
[Aim]To investigate the effects of two molluscicides,niclosamide ethanolamine salt and metaldehyde,on the gut microbi-ota structure of Pomacea canaliculata,and the responses of the gut microbiota.[Method]Healthy individuals of P.canaliculata with the body length of 25 mm to 30 mm were treated with niclosamide ethanolamine salt and metaldehyde at LC25 and LC50 and under static starvation conditions for 96 h.The survival rates and the structural changes of gut microbiota were analyzed.Illumina MiSeq high-throughput sequencing of bacterial 16S rRNA V3-V4 region was conducted for 14 gut samples of P.canaliculata.[Result]The actual mortality rates of P.canaliculata exposed to metaldehyde at LC25 and LC50 were 9.44％ and 12.22％,respectively,higher than theoreti-cal values.The actual mortality rates of P.canaliculata exposed to niclosamide ethanolamine salt at LC25 and LC50 were 3.89％-4.44％ lower than the theoretical values.Both molluscicides decreased the gut microbiota diversity.Metaldehyde reduced the species richness by 25.2％-32.0％,which significantly exceeded the reduction(7.8％-15.7％)caused by niclosamide ethanolamine salt.The β-diversity analysis showed minor differences of the gut microbiota structure under niclosamide ethanolamine salt treatment.However,metaldehyde drastically altered the gut microbiota structure,decreasing the relative abundance of Cloacibacterium from 61.49％to 7.78％-11.92％and increasing the relative abundance of Bacteroides to 21.46％-62.21％.Moreober,distinct gut microbiota variations were observed af-ter treatments with metaldehyde at LC25 and LC50.[Conclusion]Metaldehyde causes greater disruption to the gut microbiota balance of P.canaliculata than niclosamide ethanolamine salt,especially affecting key genera(Cloacibacterium,Lactococcus,and Bacteroides)involved in the balance.Gender-specific responses to the two molluscicides are observed.Female snails maintain higher levels of bene-ficial bacteria in the gut and stronger molluscicide tolerance than male snails.Both male and female P.canaliculata may alleviate mol-luscicide stress by enhancing gut microbiota metabolism or regulating host immune functions.关键词
杀螺胺乙醇胺盐/四聚乙醛/致死浓度/福寿螺/肠道微生物/高通量测序Key words
niclosamide ethanolamine salt/metaldehyde/lethal concentration/Pomacea canaliculata/gut microbiota/high-throughput sequencin引用本文复制引用
王梓孚,杨海君,万自学,黎标..杀螺胺乙醇胺盐和四聚乙醛不同致死浓度对福寿螺肠道微生物群落特征的影响[J].生物安全学报（中英文）,2025,34(2):145-154,10.基金项目
省市联合基金项目(2022JJ50247) （2022JJ50247）
