山西大学学报（自然科学版）2025，Vol.48Issue(3)：617-625,9.DOI:10.13451/j.sxu.ns.2025019
具有双重猝灭机制的高灵敏荧光探针对锌离子的检测
A Highly Sensitive Fluorescent Probe Utilizing a Dual Quenching Mechanism for Zn2+Detection
摘要
Abstract
To enhance the sensitivity of fluorescence probes for the detection of Zn2+,this study introduces an OFF-ON Zn2+detection strategy.This approach utilizes a dual quenching mechanism involving fluorescein ring closure-opening and(E)-4-((4-(dimethylami-no)phenyl)diazenyl)benzoic acid(DABCYL)as a dual quencher.The primary objective is to address the issue of high background fluorescence associated with bis(pyridin-2-ylmethyl)amine(DPA)based Zn2+recognition groups in various fluorescence probes.The Zn2+fluorescence probe 4',5'-bis((bis(pyridin-2-ylmethyl)amino)methyl)-2',7'-dichloro-3'-hydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-6'-yl(E)-4-((4-(dimethylamino)phenyl)diazenyl)benzoate(FL-ZJ)was synthesized using a fluorescein precursor mole-cule,with DPA serving as the recognition group and DABCYL functioning as the dual quencher.The fluorescence response of FL-ZJ to Zn2+was investigated through fluorescence spectroscopy.Results indicated that FL-ZJ exhibited minimal fluorescence activity at 526 nm in the absence of Zn2+.Upon introduction of Zn2+,the fluorescence intensity at 526 nm increased to approximately 17.4-fold,ac-companied by a color change from colorless to yellow.The detection limit for Zn2+using this probe was determined to be 14.6 nmol/L.Further analysis via Job's Plot experiments and high-performance liquid chromatography revealed that coordination-induced ring opening of the fluorescein moiety and intramolecular elimination of the quenching group facilitated the selective detection of Zn2+.关键词
背景荧光/OFF-ON/锌离子/痕量Key words
background fluorescence/OFF-ON/zinc ion/trace分类
数理科学引用本文复制引用
高巍,殷凯越,王泽晋,王清明,苏静,侯效佳,袁彩霞..具有双重猝灭机制的高灵敏荧光探针对锌离子的检测[J].山西大学学报（自然科学版）,2025,48(3):617-625,9.基金项目
山西省基础研究计划资助项目(202303021211017) （202303021211017）
山西省科技创新项目(2023L334) （2023L334）
长治学院校本应用教材建设项目(XN0329) （XN0329）
