陕西师范大学学报（自然科学版）2025，Vol.53Issue(3)：66-82,17.DOI:10.15983/j.cnki.jsnu.2025206
复方菌草灵芝颗粒的水提工艺及其质量标准研究
Research on water extraction process of compound Juncao Ganoderma lucidum granules and the formulation of quality standard
摘要
Abstract
In order to explore the water extraction process and quality standard of compound Juncao Ganoderma lucidum granules,liquid extract yield and liquid extract polysaccharide content were taken as indexes,the effects of extraction temperature,extraction time,ratio of material to liquid,extraction times and soaking time on extraction yield and polysaccharide content were investigated by single factor and response surface experiments.The particle size,moisture content,weight loss on drying,loading difference,solubility,microbial limit and heavy metal content of granules were detected according to the 2015 edition of Pharmacopoeia of the People's Republic of China and the national standard.The raw materials of Juncao Ganoderma lucidum,Perilla frutescens and selenium-enriched tea in compound Juncao Ganoderma lucidum granules were identified by thin layer chromatography,and the contents of monosaccharides and tea polyphenols in compound Juncao Ganoderma lucidum granules were determined through high performance liquid chromatography.The results showed that the optimal extraction conditions were as follows:extraction temperature 80 ℃,extraction time 2.5 h,ratio of material to liquid(g/mL) 1∶25,two times of extraction,and immersion time 6 h.The particle size,water content,solubility,weight loss on drying and loading difference of the granules reached the requirements of the 2015 edition of Pharmacopoeia of the People's Republic of China.The microbial limit and heavy metal content of the granules met the requirements of the national standard,and the average selenium content was 0.058 3 mg/kg.The TLC bands of Juncao Ganoderma lucidum,Perilla frutescens and selenium-enriched tea were clear and well separated,with no interference from the negative control.The contents of mannose,rhamnose,glucose,galactose,arabinose,epigallocatechin gallate,epicatechin gallate,catechin,gallic acid in compound Juncao Ganoderma lucidum granules were 1.310,1.166,8.708,1.265,0.824,5.79,2.58,1.72 and 1.67 mg/g,respectively.The optimized water extraction process of the compound Juncao Ganoderma lucidum granules is stable and reliable,all the indexes of the granules meet the requirements,and the established quality standard for the water extraction process of compound Juncao Ganoderma lucidum granules is feasible.关键词
菌草灵芝/水提工艺/薄层色谱/高效液相色谱/质量标准Key words
Juncao Ganoderma lucidum/water extraction process/thin layer chromatography/high performance liquid chromatography/quality standard分类
轻工纺织引用本文复制引用
张桂清,梁志豪,刘青,吴小平,罗海凌,姚俊新,刘斌,林占熺,江书松..复方菌草灵芝颗粒的水提工艺及其质量标准研究[J].陕西师范大学学报（自然科学版）,2025,53(3):66-82,17.基金项目
福建省科学技术厅高校产学合作项目(2015Y4001) （2015Y4001）
福建农林大学创新专项基金(CXZX2020147C) （CXZX2020147C）
福建省科学技术厅引导性项目(2022N0011) （2022N0011）
