陕西师范大学学报（自然科学版）2025，Vol.53Issue(3)：83-93,11.DOI:10.15983/j.cnki.jsnu.2025207
玉米质膜Na+/H+反向转运蛋白NHX7对根际土壤微生物群落的影响
Effect of maize plasma membrane Na+/H+antiporter NHX7 on rhizosphere microbial communities
摘要
Abstract
Roots are essential multifunctional plant organs involved in water and nutrient uptake,metabolite storage,anchorage,mechanical support and interaction with the soil environment.Root system architecture(RSA)plays an important role in plant adaptation to abiotic adversity stresses,while it is also influenced by genetic factors and microbial community composition.The SOS1 gene is a core component in the plant salt tolerance signaling pathway and plays an important role in plant salt tolerance.However,whether it affects rhizosphere microorganism has not been reported.Field planting experiment found that the maize nhx7-1(sos1)mutant exhibited severe growth defects in both plant height and root system compared to wild-type B73 when grown in Kaifeng field,Henan Province,whereas its plant and root growth phenotypes were similar to those of B73 when grown in Sanya field,Hainan Province.Transcriptome sequencing combined with KEGG enrichment analysis revealed that the differentially expressed genes in the root system between nhx7-1 and B73 were mainly enriched in the pathways of secondary metabolite biosynthesis,amino acid metabolism and flavonoid synthesis.The sequencing results of bacteria 16S rDNA and fungus ITS showed that the diversity of rhizosphere bacterial and fungal communities between nhx7-1 and B73 demonstrated different patterns,and the root morphology was greatly influenced by the rhizosphere bacterial community,with a significantly positive correlation between the core node ASV565 and root length.The results suggest that root secondary metabolites may be involved in the plasticity process of root conformation by influencing rhizosphere microorganisms,especially bacteria.关键词
根系结构/SOS1基因/转录组测序/次生代谢物/根际微生物Key words
root system architecture/SOS1 gene/transcriptome sequencing/secondary metabolites/rhizosphere microorganisms分类
生物科学引用本文复制引用
魏锋,郭康,赵依格,时玉,胡筑兵..玉米质膜Na+/H+反向转运蛋白NHX7对根际土壤微生物群落的影响[J].陕西师范大学学报（自然科学版）,2025,53(3):83-93,11.基金项目
海南省科技计划三亚崖州湾科技城自然科学基金联合项目(2021JJLH0008) （2021JJLH0008）
