陕西师范大学学报（自然科学版）2025，Vol.53Issue(3)：94-101,8.DOI:10.15983/j.cnki.jsnu.2025208
新冠非结构蛋白nsp9细胞定位及其与宿主蛋白互作分析
Analysis of cellular localization and host protein interactions involving the non-structural protein nsp9 of SARS-CoV-2
摘要
Abstract
Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)has had a profound and severe impact on human health and socio-economic structures.Dissecting the core aspects of the replication mechanism of this virus is particularly important for the development of effective prevention and control strategies.As a key single-stranded nucleic acid binding protein,nsp9 plays a pivotal role in the virus life cycle.Overexpression of green fluorescent protein(GFP)-fused nsp9 in cells and observation with confocal laser microscopy revealed that nsp9 is mainly localized in nucleus.The interactions between nsp9 and several intracellular proteins,including YWHAB and TRAF2,were initially identified by immunoprecipitation combined with mass spectrometry,and were verified by immunocoprecipitation and pull-down assay.In addition,the potential effects of nsp9 on the biological characteristics of host cells were investigated by CCK8 cell viability detection,EdU cell proliferation experiment and TUNEL apoptosis test,and it was found that overexpression of nsp9 could not significantly affect the normal proliferation of cells or induce apoptosis.These findings offer a foundation for subsequent comprehensive investigation into the interaction between nsp9 and host protein as well as its molecular mechanism in SARS-CoV-2 infection.关键词
SARS-CoV-2/非结构蛋白/nsp9/互作蛋白Key words
SARS-CoV-2/non-structural protein/nsp9/interaction proteins分类
农业科技引用本文复制引用
张倡珲,代玉涵,王应归,王美林,赵一洋..新冠非结构蛋白nsp9细胞定位及其与宿主蛋白互作分析[J].陕西师范大学学报（自然科学版）,2025,53(3):94-101,8.基金项目
四川省科技厅计划项目(2023JDKP0109) （2023JDKP0109）
中央高校基本科研业务费专项基金(ZYN2024052) （ZYN2024052）
