陕西师范大学学报（自然科学版）2025，Vol.53Issue(3)：102-114,13.DOI:10.15983/j.cnki.jsnu.2025209
盐胁迫下花生CBL基因家族鉴定及表达模式分析
Identification and expression analysis of CBL genes in peanut(Arachis hypogaea L.)under salt stress
摘要
Abstract
Calcineurin B-like proteins(CBLs)are involved in plant-specific Ca2+signaling as calcium (Ca) sensors and are essential for plant response to abiotic stress.Peanut is an important oilseed crop,and salt stress seriously affects the growth and development of peanut and its seed quality.At present,the response of peanut CBL genes to salt stress is unknown.In this study,members of the CBL gene family were identified from the peanut genome-wide database using bioinformatics methods and were analyzed for physicochemical properties,gene structure,phylogenetic tree and colinearity.The study found that there are 89 CBL genes in the peanut genome distributed on 19 chromosomes.Analysis of the physicochemical properties of the proteins showed that peanut CBLs are mainly composed of acidic amino acids.Subcellular localization predictions revealed that most of the CBLs in peanut are localized in the nucleus.To investigate the response of peanut CBL genes to salt stress,the expression patterns of five CBL genes were analyzed under salt stress.After treating with 200 mmol/L NaCl for 3 h,the expression of AhCBL46.1 was significantly increased,and the expressions of AhCBL4,AhCBL33 and AhCBL66 were significantly increased after 12 h of NaCl treatment.However,AhCBL61.3 showed a negative response to salt stress,suggesting that the peanut CBL genes play an important role in salt stress regulation.This research lays the groundwork for further exploration into the functionality of AhCBL genes.关键词
花生/CBL/基因家族/理化特性/盐胁迫Key words
Arachis hypogaea L./CBL/gene family/physicochemical property/salt stress分类
农业科技引用本文复制引用
程在,朱秀,朱斌,谷雷,曾拓,王洪程,杜旭烨..盐胁迫下花生CBL基因家族鉴定及表达模式分析[J].陕西师范大学学报（自然科学版）,2025,53(3):102-114,13.基金项目
贵州师范大学黔师新苗项目([2022]B10号,[2022]B30号,[2022]15号) （[2022]B10号,[2022]B30号,[2022]15号）
