实用医学杂志2025，Vol.41Issue(9)：1273-1284,12.DOI:10.3969/j.issn.1006-5725.2025.09.002
IQGAP2在肾细胞癌中的表达以及对肾癌细胞恶性生物学行为的影响
The expression of IQGAP2 in renal cell carcinoma and its effect on the malignant biological behavior of renal cell carcinoma
摘要
Abstract
Objective To investigate the expression pattern of IQGAP2 in renal cell carcinoma tissues and cell lines,and to analyze its effects on the proliferation and migration of renal cell carcinoma cells.Methods Firstly,GEO database was used to screen differentially expressed genes between renal cell carcinoma tissues,and GEPIA,TIMER2.0 were used to analyze the expression level of IQGAP2 in renal cell carcinoma tissue.Subse-quently,knockdown(siRNA)and overexpression plasmids of IQGAP2 were constructed and transfected into ACHN and 786-O cell lines to perform a series of functional experiments to evaluate the effect of IQGAP2 on the malignant biological behavior of renal carcinoma cells.qRT-PCR and Western Blot were used to detect the expres-sion of EMT(epithelial-mesenchymal transition)related proteins after knockdown and overexpression of IQGAP2.Results In renal cell carcinoma tissues,the relative expression of IQGAP2 was significantly lower than in adja-cent normal tissues(P<0.001).Transfection of si-IQGAP2 in ACHN and 786-O cells effectively downregulated the mRNA and protein expression levels of IQGAP2(P<0.01),while transfection with an overexpression plasmid significantly upregulated its mRNA and protein expression(P<0.001).Further studies revealed that overexpression of IQGAP2 significantly inhibited the proliferation(P<0.05)and migration(P<0.01)of ACHN and 786-O cells,whereas knockdown of IQGAP2 enhanced their proliferation(P<0.05,P<0.001)and migration(P<0.01).Through qRT-PCR and Western blot analyses of EMT-related proteins,it was found that reduced expression of IQGAP2 promoted the epithelial-mesenchymal transition(EMT)process in renal cancer cells.Conclusions The expression of IQGAP2 is low in renal cell carcinoma tissues and cells,and the decrease of the expression level can promote the EMT process of renal cell carcinoma cells,and then enhance the proliferation and migration of renal cell carcinoma cells.IQGAP2 plays an important tumor suppressor role in renal cell carcinoma.关键词
肾细胞癌/IQ基序GTPase激活蛋白2/786-O/ACHN/生物学行为Key words
renal cancer/IQGAP2/786-O/ACHN/biological behavior分类
临床医学引用本文复制引用
陈浩,牛云峰,王琦,吕婷,李涛,曾琨鹏,樊博..IQGAP2在肾细胞癌中的表达以及对肾癌细胞恶性生物学行为的影响[J].实用医学杂志,2025,41(9):1273-1284,12.基金项目
国家自然科学基金面上项目(编号:81572441) （编号:81572441）
河北省医学科学研究课题计划(编号:20250109) （编号:20250109）
