实用医学杂志2025，Vol.41Issue(9)：1319-1326,8.DOI:10.3969/j.issn.1006-5725.2025.09.007
LncRNA MEG3通过调控COX-2/PGE2/VEGF信号通路对早期糖尿病大鼠视网膜的保护作用
Protective effect of LncRNA MEG3 on diabetic retinopathy in rats by regulating COX-2/PGE2/VEGF sig-naling pathway
摘要
Abstract
Objective To investigate the protective effect of LncRNA MEG3 on the retina in early-stage diabetic rats through regulation of the COX-2/PGE2/VEGF signaling pathway.Methods 50 male SD rats of SPF grade were selected for the study.Among them,10 rats were assigned to the control group,while 40 rats were used to establish diabetic retinopathy models.A total of 32 rats successfully underwent modeling and were subsequently divided into four groups(n=8 per group):model group,negative control group,MEG3 overexpression group,and MEG3 overexpression+COX-2 inhibitor group.Histopathological changes,vascular permeability,glucose and lipid metabolism,inflammatory factors,oxidative stress indices,PGE2 levels,as well as the relative mRNA and protein expression levels of COX-2 and VEGF were evaluated in each group.Results Compared with the control group,HDL-C,CAT,GSH-PX,and SOD levels were significantly decreased,whereas the mRNA and protein expression levels of vascular permeability,TG,TC,LDL-C,IL-6,IL-1β,TNF-α,MDA,PGE2,COX-2,and VEGF were significantly increased in the model group(P<0.05).Compared with the negative control group,HDL-C,CAT,GSH-PX,and SOD levels were significantly increased in the MEG3 overexpression group,while the mRNA and protein expression levels of vascular permeability,TG,TC,LDL-C,IL-6,IL-1β,TNF-α,MDA,PGE2,COX-2,and VEGF were significantly decreased(P<0.05).Compared with the MEG3 overexpression group,HDL-C,CAT,GSH-PX,and SOD levels were further increased in the MEG3 overexpression+COX-2 inhibitor group,and the mRNA and protein expression levels of vascular permeability,TG,TC,LDL-C,IL-6,IL-1β,TNF-α,MDA,PGE2,COX-2,and VEGF were further decreased(P<0.05).Conclusion LncRNA MEG3 is capable of regulating the COX-2/PGE2/VEGF pathway,enhancing glucose and lipid metabolism in rats,suppressing the expression of inflammatory factors,attenuating stress responses,and alleviating diabetic retinopathy.关键词
糖尿病视网膜病变/长链非编码RNA母系表达基因3/视网膜环氧合酶-2/前列腺素E2/血管内皮生长因子信号通路/血管通透性Key words
diabetic retinopathy/long non-coding rna maternal expression gene 3/retinal cyclooxy-genase-2/prostaglandin e2/vascular endothelial growth factor signaling pathway/vascular permeability分类
临床医学引用本文复制引用
陈梅,李宗智,秦学维,王利民,郑丽..LncRNA MEG3通过调控COX-2/PGE2/VEGF信号通路对早期糖尿病大鼠视网膜的保护作用[J].实用医学杂志,2025,41(9):1319-1326,8.基金项目
国家自然科学基金(地区科学基金)项目(编号:82060886) （地区科学基金）
贵州省中医药管理局中医药、民族医药科学技术研究专项课题(编号:QZYY-2019-005) （编号:QZYY-2019-005）
