实用医学杂志2025，Vol.41Issue(10)：1540-1547,8.DOI:10.3969/j.issn.1006-5725.2025.10.016
血清纤维连接蛋白在非小细胞肺癌患者中的表达水平及其临床应用价值
The expression level of serum fibronectin in patients with non-small cell lung cancer and its clinical appli-cation value
摘要
Abstract
Objective To investigate the expression levels of serum fibronectin(FN)in patients with non-small cell lung cancer(NSCLC)and evaluate its clinical diagnostic significance.Methods Firstly,the gene and tissue protein expression levels of FN in NSCLC were assessed using the TIMER2.0 database,GTEx database,and Human Protein Atlas(HPA)database.Secondly,from January 2021 to June 2023,a total of 154 patients with lung space-occupying lesions or shadows at the First Affiliated Hospital of Anhui University of Chinese Medicine were enrolled.Based on medical history,clinical manifestations,imaging tests,and pathological histological find-ings,these patients were categorized into two groups:81 patients with NSCLC and 73 patients with benign lung diseases.The serum concentration of FN was measured by immunoturbidimetry,while the serum levels of CEA,SCC,and CYFRA21-1 were determined using chemiluminescence.Results The bioinformatics analysis results demonstrated that the gene expression and tissue protein levels of FN were significantly reduced compared to normal tissues.In serological evaluations,serum FN concentrations in patients with advanced NSCLC(Stage Ⅲ-Ⅳ)and early-stage NSCLC(Stage Ⅰ-Ⅱ)(249.50 ng/mL and 305.00 ng/mL,respectively)were markedly lower than those observed in the benign lung disease group(429.16 ng/mL)(P<0.001).Univariate and multivariate logistic regression analyses indicated that serum FN could serve as a significant predictor for the presence of NSCLC.The area under the receiver operating characteristic curve(ROC)for serum FN in diagnosing early-stage NSCLC was 0.790,which outperformed CEA(0.618),SCC(0.653),and CYFRA21-1(0.601).The optimal critical concen-tration of serum FN for distinguishing early-stage NSCLC from benign diseases was determined to be 347.6 ng/mL,with a sensitivity of 71.93％,surpassing that of CEA(19.30％),SCC(12.28％),and CYFRA21-1(8.77％).Conclusion Serum FN is significantly downregulated in NSCLC and may serve as a potential biomarker for the early diagnosis of NSCLC.关键词
非小细胞肺癌/纤维连接蛋白/癌胚抗原/鳞状细胞癌抗原/细胞角蛋白19片段Key words
NSCLC/Fibronectin/CEA/SCC/CYFRA21-1分类
医药卫生引用本文复制引用
王庆保,陈首慧,刘亚婷,朱俊,张波克..血清纤维连接蛋白在非小细胞肺癌患者中的表达水平及其临床应用价值[J].实用医学杂志,2025,41(10):1540-1547,8.基金项目
安徽省自然科学基金面上项目(编号:2308085MH291) （编号:2308085MH291）
安徽省高等学校自然科学研究重点项目(编号:2024AH051000) （编号:2024AH051000）
安徽省卫生健康委科研项目(编号:AHWJ2023BAa20124) （编号:AHWJ2023BAa20124）
