生殖医学杂志2025，Vol.34Issue(5)：631-638,8.DOI:10.3969/j.issn.1004-3845.2025.05.009
活性氧簇在小鼠子宫内膜生理修复过程中的动态变化研究
Dynamic changes of reactive oxygen species in physiological repair process of mouse endometrium
摘要
Abstract
Objective:To investigate the dynamic changes and functional role of reactive oxygen species(ROS)during endometrial repair. Methods:A mouse model of menstruation-like endometrial injury was established.Uterine tissues were collected at 24,36,and 48 hours after progesterone withdrawal during the repair process.ROS levels were assessed using dihydroethidium(DHE)staining,and a hydroxyl radical assay kit was employed to evaluate the endometrial tissue's capacity to inhibit hydroxyl radicals.Real-time quantitative PCR(qRT-PCR)was used to measure mRNA levels of the cell proliferation marker Ki67 and the oxidative stress-related factor Nox4.Additionally,immunohistochemistry(IHC)was performed to detect the expression and localization of the proliferation marker proliferating cell nuclear antigen(PCNA). Results:At 24 hours post-progesterone withdrawal,the endometrium exhibited a fully disintegrated necrotic phenotype.By 36 hours,the necrotic tissue had gradually detached,coinciding with basal lamina repair.By 48 hours,the uterine morphology closely resembled that of a normal uterus.ROS levels and the antioxidant capacity(hydroxyl radical suppression)were significantly elevated at 36 hours post-progesterone withdrawal when compared with 24 hours(P＜0.05),followed by a rapid decline to the lowest levels between 36 and 48 hours(P＜0.05).The qRT-PCR analysis showed that Ki67 and Nox4 mRNA levels at 36 hours post-progesterone withdrawal were significantly higher than those at both 24 and 48 hours(P＜0.05).Similarly,IHC results demonstrated that PCNA expression peaked at 36 hours post-progesterone withdrawal,showing significantly higher levels than those at 24 and 48 hours(P＜0.05).At 36 hours post-progesterone withdrawal,PCNA-positive signals were distributed in the nuclei of luminal epithelium,vascular endothelium,glands,and stromal cells,whereas at 48 hours,they were confined to the nuclei of luminal epithelium and stromal cells. Conclusions:During the physiological repair of the endometrium in a mouse menstrual-like model,ROS levels which increased firstly and then decreased were closely associated with endometrial cell proliferation during the repair process,highlighting the critical biological role of ROS in endometrial regeneration.关键词
活性氧簇/子宫内膜修复/细胞增殖/氧化应激/NOX4Key words
Reactive oxygen species/Endometrial repair/Cell proliferation/Oxidative stress/NOX4分类
基础医学引用本文复制引用
王思雨,张昕,龙泽怡,安苒,陈西华,王树芳,徐祥波..活性氧簇在小鼠子宫内膜生理修复过程中的动态变化研究[J].生殖医学杂志,2025,34(5):631-638,8.基金项目
中央级公益性科研院所基本科研业务费专项(2023GJZ04,2023GJZD0105) （2023GJZ04,2023GJZD0105）
