天津中医药大学学报2025，Vol.44Issue(4)：317-323,7.DOI:10.11656/j.issn.1673-9043.2025.04.06
益气清肺颗粒干预对慢性疲劳综合征模型小鼠的药效作用研究
Evaluation of the pharmacological effect of Yiqi Qingfei Granules intervention on chronic fatigue syndrome model mice
摘要
Abstract
[Objective]To explore the anti-fatigue effect of Yiqi Qingfei Granules(YQQF)on chronic fatigue model mice.[Methods]Male balb/c mice were randomly divided into the control group,the model group,YQQF low dose group(2.4 g/kg),YQQF middle dose group(4.8 g/kg),YQQF high dose group(9.6 g/kg).Mice in the control and model groups were given saline,and other mice were given YQQF.One hour after intragastric administration,all mice except those in the control group were subjected to swimming training for 2 hours,and the process lasted for 6 weeks.All mice were killed 24 hours after the last exhaustive swimming training.Forced swimming test and rotarod test were carried out to evaluate endurance.Contents of glycogen and adenosine triphosphate(ATP),and levels of serum urea nitrogen(BUN)and lactate(LA)were detected by biochemical assay kits.The concentrations of tryptophan and serotonin(5-HT)in brain tissue were tested by ELISA.The expression levels of Calcium/Calmodulin-dependent Protein Kinase Kinase 2(CaMKK2),Adenosine 5'-monophosphate(AMP)-activated protein kinas(AMPK),p-AMPK,peroxisome proliferator-activated receptor gamma coactivator 1α(PGC-1α)and Sirtuin 3(SIRT3)in gastrocnemius were assayed by Western blot.[Results]Compared with the model group,YQQF significantly prolonged the exhaustion swimming time and rotation time(P＜0.05 or P＜0.01),increased the glycogen content and ATP level(P＜0.05),up-regulated p-AMPK,PGC-1α and SIRT3 protein levels(P＜0.05 or P＜0.01),while the levels of LA,BUN,tryptophan and 5-HT were decreased(P＜0.05 or P＜0.01).However,there was no significant difference in CaMKK2 expression levels of gastrocnemius among groups(P＞0.05).[Conclusion]YQQF may exert therapeutic effects on chronic fatigue syndrome by activating the gastrocnemius AMPK/PGC-1α/SIRT3 signaling pathway and improving energy metabolism.关键词
益气清肺颗粒/慢性疲劳综合征/能量代谢/5-羟色胺/AMPK/PGC-1α/SIRT3信号通路Key words
Yiqi Qingfei Granule/chronic fatigue syndrome/energy metabolism/5-HT/AMPK/PGC-1α/SIRT3 signaling pathway分类
中医学引用本文复制引用
高盼微,张晗,苗琳,柴丽娟,徐兴娣,罗子娟,常慧琳,王娜,陆兴玥,袁庆,丁辉,张晓光..益气清肺颗粒干预对慢性疲劳综合征模型小鼠的药效作用研究[J].天津中医药大学学报,2025,44(4):317-323,7.基金项目
国家重点研发项目(2023YFC3504302) （2023YFC3504302）
天津市科技计划项目(22ZXGBSY00020). （22ZXGBSY00020）
