摘要
Abstract
[Objective]Looking for research hotspots in Zhisou Powder(ZSP).To study the regulatory effect of ZSP on airway aquaporin(AQP)1,5 and mucin(MUC)5AC induced by neutrophil elastase(NE).[Methods]NCI-H292 cells were divided into control group,NE group,and ZSP intervention group.The content of AQP1 mRNA,AQP5 mRNA,and MUC5AC mRNA was detected using qPCR method,Western blot method was used to detect AQP1 and AQP5 proteins,and ELISA method was used to detect MUC5AC protein.[Result]1)Research hotspot disease:cough,chronic cough,and exogenous cough,the characteristics of traditional Chinese medicine is syndrome differentiation.2)NE induced NCI-H292:compared with the control group,the NE group showed a decrease in AQP1 mRNA and AQP5 mRNA,an increase in MUC5AC mRNA,a decrease in AQP5 protein expression,an increase in MUC5AC protein,and no significant changes in AQP1 protein.Compared with the NE group,after treatment with ZSP,MUC5AC mRNA significantly decreased,with a downward trend in MUC5AC,and no significant changes in AQP1 and AQP5.[Conclusion]ZSP can downregulate the high expression of MUC5AC mRNA in NCI-H292 cells induced by NE,and has a downward trend towards the high expression of MUC5AC protein.This suggests that the mechanism of action of the anti-cough,volatile and expectorant effects may be mainly related to the regulation of mucin,but the relevant conclusions still need to be further validated through clinical and animal experiments.关键词
止嗽散/NCI-H292 细胞/水通道蛋白1/水通道蛋白 5/黏蛋白 5ACKey words
Zhisou Powder/NCI-H292 cell/aquaporin 1/aquaporin 5/mucin 5AC分类
中医学
