西安交通大学学报（医学版）2025，Vol.46Issue(3)：448-454,7.DOI:10.7652/jdyxb202503010
非酒精性脂肪肝患者的肠道微生物特征
Gut microbiota characteristics in patients with nonalcoholic fatty liver disease
摘要
Abstract
Objective To analyze the changes of intestinal microbiota in patients with nonalcoholic fatty liver disease(NAFLD)and healthy controls so as to explore the relationship between intestinal microbiota and NAFLD.Methods A total of 353 samples from the National Center for Biotechnology Information were included,including 110 healthy controls and 243 NAFLD patients(127 NASH and 116 NAFLD).Macrogenome sequencing data were extracted to obtain data on intergroup flora diversity changes and analyze the potential correlation between alterations in gut microbes and NAFLD.Results Alpha diversity analysis showed no significant differences in Invsimpson index(P=0.57)or Simpson index(P=0.57)between NAFLD patients and healthy controls,while there was a trend of decreasing Shannon index(P=0.084),but with no significant difference.β-diversity analysis showed significant differences between the two groups(P=0.002).Species composition analysis showed that at the genus level,NAFLD patients and healthy controls had the same species types,with Bacteroidetes and Firmicutes being the dominant phyla in both groups.However,compared to Control group,NAFLD group had significantly lower Firmicutes,Synergistetes,and Candidatus_Melainabacteria.At the species level,there were significant differences between NAFLD group and Control group for 10 bacterial species,including Bacteroides_uniformis and Faecalbacterium_prausnitzil.Intergroup difference analysis showed that Phocaeicola dorei,Parabacteroides_distasonis,Bilophila_wadsworthia,and Dysosmobacter_welbionis were significantly enriched in NAFLD patients(LDA score＞2.0,P＜0.05),while Prevotella_stercorea,Faecalibacterium prausnitzii,and others were negatively associated with NAFLD.A disease detection model was constructed based on metagenomic data,and 26 intestinal microorganisms related to NAFLD were selected,including Pyramidobacter piscolens and Citrobacter freundii,etc.The diagnostic accuracy of NAFLD was judged by ROC curve discriminant model,and the AUC of the ROC curve was 0.743 8,indicating that the model has a certain accuracy in diagnosing NAFLD.Conclusion This study revealed differences in gut microbial diversity between NAFLD patients and healthy controls and identified key bacterial groups associated with NAFLD.In addition,the disease detection model constructed based on metagenomic data provides a new method and idea for the diagnosis of NAFLD,which is conducive to further study of the disease and the early diagnosis and treatment of clinical patients.关键词
非酒精性脂肪肝(NAFLD)/肠道微生物/宏基因组Key words
non-alcoholic fatty liver disease(NAFLD)/gut microbiome/metagenomics分类
医药卫生引用本文复制引用
刘凯顺子,方敏,段锦花,李春娥,朱峰,赵哲..非酒精性脂肪肝患者的肠道微生物特征[J].西安交通大学学报（医学版）,2025,46(3):448-454,7.基金项目
陕西省重点研发计划项目(No.2021GXLH-Z-083),西安交通大学第一附属医院临床研究重点项目(No.XJTU1AF2021CRF-008)Supported by Shaanxi Province Key Research and Development Plan Project(No.2021GXLH-Z-083)and Key Project of Clinical Research of The First Affiliated Hospital of Xi'an Jiaotong University(No.XJTU1AF2021CRF-008) （No.2021GXLH-Z-083）
