西安交通大学学报（医学版）2025，Vol.46Issue(3)：504-510,7.DOI:10.7652/jdyxb202503018
铁自噬相关基因ELAVL1在多发性骨髓瘤中的表达及临床意义
The expression and clinical value of ferritinophagy-related gene ELAVL1 in multiple myeloma
摘要
Abstract
Objective To investigate the expression of ferritinophagy-related gene ELAV-like RNA binding protein 1(ELAVL1)in multiple myeloma(MM)and elucidate its diagnostic and prognostic value for MM.Methods First,we analyzed ELAVL1 expression level in healthy controls and MM patients using data from the GEO and TCGA databases.Subsequently,bone marrow specimens were collected from 28 newly diagnosed MM patients and 20 healthy controls,and qRT-PCR was employed to validate ELAVL1 expression.The diagnostic and prognostic potential of ELAVL1 was assessed using ROC curve analysis and Kaplan-Meier survival curves.Additionally,univariate and multivariate COX regression analyses were performed to identify independent risk factors for MM prognosis.Finally,KEGG and GO enrichment analyses were performed using the DAVID online platform.Results The level of ELAVL1 expression was significantly higher in newly diagnosed MM patients and refractory/relapsed MM patients than in the healthy controls(P＜0.001).Moreover,ELAVL1 expression was positively correlated with the International Staging System(ISS)stage of MM(P＜0.01).Furthermore,qRT-PCR validation confirmed that ELAVL1 expression was elevated in the 28 newly diagnosed MM patients compared to the 20 healthy controls(P＜0.001).ROC curve analysis demonstrated that ELAVL1 could effectively differentiate between newly diagnosed MM patients,healthy controls,and MGUS patients(P＜0.001 and P=0.000 2,respectively).Survival analysis revealed that high ELAVL1 expression was associated with shorter progression-free survival(P=0.0141)and overall survival(P=0.008 0).Univariate and multivariate COX regression analyses identified high ELAVL1 expression as an independent risk factor for poor MM prognosis(P=0.005 0).KEGG analysis suggested that ELAVL1 might be involved in the Hippo and MAPK signaling pathways.Conclusion High ELAVL1 expression in MM may serve as a biomarker for diagnosis and poor prognosis.ELAVL1 may promote MM initiation and progression via the Hippo and MAPK signaling pathways.关键词
多发性骨髓瘤(MM)/铁自噬/ELAV样RNA结合蛋白1(ELAVL1)/Hippo信号通路/MAPK信号通路Key words
multiple myeloma(MM)/ferritinophagy/ELAV-like RNA binding protein 1(ELAVL1)/Hippo signaling pathway/MAPK signaling pathway分类
医药卫生引用本文复制引用
章瑞,李琳,李乔,罗思宇,何爱丽,孔光耀,贾亚春,万冰洁,任晓敏,MUNYURANGABO Gustave,于潇,缪吉玉,张佩华,柳宏苇,杨丹..铁自噬相关基因ELAVL1在多发性骨髓瘤中的表达及临床意义[J].西安交通大学学报（医学版）,2025,46(3):504-510,7.基金项目
国家自然科学基金资助项目(No.82170140,No.82370146) （No.82170140,No.82370146）
陕西省重点研究发展计划项目(No.2024SF-YBXM-151) （No.2024SF-YBXM-151）
陕西省化学与生物基础科学研究项目(No.23JHZ007)Supported by the National Natural Science Foundation of China(No.82170140,No.82370146),Shaanxi Key Research and Develop-ment Program Project(No.2024SF-YBXM-151),and Shaanxi Basic Research Project on Chemistry and Biology(No.23JHZ007) （No.23JHZ007）
