西安交通大学学报（医学版）2025，Vol.46Issue(3)：522-531,10.DOI:10.7652/jdyxb202503021
鳖甲煎丸通过CMTM6抑制Hep3B细胞的恶性生物学行为
Biejiajian Pill inhibits the malignant biological behavior of Hep3B cells through CMTM6
摘要
Abstract
Objective To investigate the effect of Biejiajian Pill(BJJP)on malignant biological behavior of hepatocellular carcinoma Hep3B cells and its regulatory mechanism.Methods A total of 72 healthy SD rats were randomly divided into blank control(BC)group,low(0.55 g/kg),medium(1.10 g/kg)and high(2.20 g/kg)BJJP experimental group,and drug-containing serum was prepared.Hep3B cells were divided into BC group,normal rat serum treatment(NC)group,low dose BJJP(LBJJP)group,medium dose BJJP(MBJJP)group and high dose BJJP(HBJJP)group,empty plasmid(pcDNA3.1)group,and CKLF-like MARVEL transmembrane domain containing 6(CMTM6)overexpression(pcDNA3.1-CMTM6)group,and the NC+pcDNA3.1 group,MBJJP+pcDNA3.1 group,NC+pcDNA3.1-CMTM6 group and MBJJP+pcDNA3.1-CMTM6 group.The proliferation of hepatoma Hep3B cells was detected by CCK-8.The migration and invasion of hepatoma Hep3B cells were detected by Transwell assay.The expression levels of proliferation-related proteins proliferating cell nuclear antigen(PCNA),epithelial-mesenchymal transition(EMT)related proteins(E-cadherin,N-cadherin and Vimentin),and CMTM6 proteins in hepatoma Hep3B cells were detected by Western blotting experiments.Results Compared with those in BC group,there were no significant differences in the proliferation,migration and invasion abilities of Hep3B cells,or the expression levels of PCNA,EMT related proteins(E-cadherin,N-cadherin and Vimentin)and CMTM6 protein in NC group(P＞0.05).Compared with NC group,LBJJP,MBJJP and HBJJP drug-containing serum inhibited the proliferation,migration and invasion of Hep3B cells,downregulated the protein expression of PCNA;MBJJP and HBJJP upregulated the protein expression of E-cadherin.The protein expressions of N-cadherin,Vimentin and CMTM6 were downregulated,with significant differences(P＜0.05).Compared with pcDNA3.1 group,the protein expression of CMTM6,cell proliferation,migration,invasion,PCNA protein expression,N-cadherin protein expression,and Vimentin protein expression in Hep3B cells in pcDNA3.1-CMTM6 group were significantly upregulated,while the protein expression of E-cadherin was significantly downregulated(P＜0.05).Compared with NC+pcDNA3.1 group,the proliferation,migration and invasion abilities of Hep3B cells in MBJJP+pcDNA3.1 group were decreased,the expression levels of PCNA,Vimentin and N-cadherin protein were decreased,while the expression level of E-cadherin protein was increased.Compared with NC+pcDNA3.1-CMTM6 group,MBJJP+pcDNA3.1-CMTM6 group had the same results in the proliferation,migration and invasion abilities of Hep3B cells and the protein expression levels of PCNA,Vimentin,N-cadherin and E-cadherin.The differences were statistically significant(all P＜0.05).Conclusion BJJP may inhibit the proliferation,migration,invasion and EMT of hepatoma Hep3B cells by regulating the expression of CMTM6.关键词
鳖甲煎丸(BJJP)/肝细胞癌(HCC)/上皮-间充质转化(EMT)/趋化素样因子超家族成员6(CMTM6)/迁移/侵袭Key words
Biejiajian Pill(BJJP)/hepatocellular carcinoma(HCC)/epithelial-mesenchymal transition(EMT)/CKLF-like MARVEL transmembrane domain containing 6(CMTM6)/migration/invasion分类
医药卫生引用本文复制引用
李尹凡,徐隽婕,兰涛,李玉凤,李鑫,张荣花,黄金平,朱丽华,章广玲..鳖甲煎丸通过CMTM6抑制Hep3B细胞的恶性生物学行为[J].西安交通大学学报（医学版）,2025,46(3):522-531,10.基金项目
河北省自然科学基金项目(No.H2023209047,No.H2024209077) （No.H2023209047,No.H2024209077）
河北省中央引导地方科技发展资金项目(No.246Z7720G) （No.246Z7720G）
河北省创新能力提升计划—高水平人才团队建设专项(No.205A7701H)Supported by the Natural Science Foundation of Hebei Province(No.H2023209047,No.H2024209077),the Central Guidance on Local Science and Technology Development Fund of Hebei Province(No.246Z7720G),and the Hebei Province Innovation Ability Improvement Plan—High-level Talent Team Construction Project(No.205A7701H) （No.205A7701H）
